Microsoft PowerPoint Presentation

10/20/99


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Table of Contents

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Principles of Flow Cytometry

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What do we measure? (parameters)

Isometric Plot

Light Scatter - e.g. Blood

Electronic gating

Immunophenotyping

Fluorescence - e.g. Monoclonal Antibodies

Production of monoclonal antibodies

Direct staining

Indirect staining

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Oxidative Reactions

Cellular Functions

Cell Cycle

The Cell Cycle

A typical DNA Histogram

Flow Cytometry of Apoptotic Cells

Analyzing the DNA Histogram

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From Duque et al, Clin.Immunol.News.

Multi-color studies generate a lot of data

Typical Assays

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 Author: J. Paul Robinson

Email: jpr@flowcyt.cyto.purdue.edu

Home Page: ../../../../educate1.htm