Immature Reticulocyte Fraction (IRF)Thiazole Orange by Flow Cytometry

• IRF = #HFR/#Retics

• Data Analysis

  • exclude nucleated cells (nRBCs, PMNS, lymphs)
  • exclude platelets
  • define IRF region
  • define retics

RBCs

Reticulocytes

Platelets

Nucleated

Cells

IRF

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