1. Mechanics of a flow cell
2. How foward angle light scatter is collected
3. How 90 degree light scatter is collected
4. Fluorescence detection in the flow cell
5. Fluoresecence activated cell sorting in the flowcytometer
6. Optical filters - an example of a dichroic filter
7. Mechanism of a band pass filter
8. Examples of long and short pass filters
9. Diagramatic representation of the optic layout of a flow cytometer
10. Common excitation and emission spectra of various fluorescent probes
11. Excitation and emission spectra of propidium iodide
12. Excitation and emission spectra of FITC
13. Excitation and emission spectra of PE
14. Excitation and emission spectra of APC
15. Diagram of typical research cytometer (Coulter 753)
16. Diagram of typical clinical cytometer
17. Diagram of microscope and its optics
