PPT Slide
Purdue University Cancer Center & Purdue University Cytometry Laboratories
What can Flow Cytometry Do?
Introductory Terms and Concepts
Concepts
Instrument Components
Arc Lamp Excitation Spectra
Lasers
Optical Collection systems
Elite Cytometer with 4 Lasers
Coulter Optical System – Elite/Altra
Fluidics
Fluorescence
Flow cytometry measurements
Data Presentation Formats
Data Analysis Concepts
The Cell Cycle
A DNA histogram
A typical DNA Histogram
Labeling Strand Breaks with dUTP [Fluorescein-deoxyuridine triphosphate (dUTP)]
Scatter Pattern of Human leukocytes
Three Color Lymphocyte Patterns
Live cell/dead cell PI Fluorescence
Oxidative Reactions
Calcium Flux
Membrane Potential
Summary
Email: jpr@flowcyt.cyto.purdue.edu
Home Page: /
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