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Assays in Multi Drug Resistance Detection,
Modulation, and Heterogeneity
Michigan Cancer Foundation 110 East Warren Detroit, MI 48201Laboratory Profile
Head: Awtar Krishan, Scientific Director
1. Facilities: Labs are located in Michigan Cancer Foundation, a Wayne State University Medical School affiliate. Analytical Cytometry facility includes instrumentation labs, computer facility, tissue culture facility, space for visiting scientists.
2. Instrumentation:
Flow cytometry: Becton-Dickinson FACStar cell sorter with innova 90 argon laser; EPICS 753 dual laser cell sorter with 2 innova 90 argon lasers and CR559 dye (Rhodamine 6G) laser. Also available is a Becton-Dickinson FACScan 5-parameter flow cytometer.
Other major items: Olympus and Leitz phase/fluorescence microscopes equipped with CCD, Video, and standard camera systems. Perkin Elmer LS-3B fluorescence spectrometer, SLM-Aminco 8000C dual beam fluorescence spectrometer, Millipore Cytofluor 2300 multi-position plate reader, Zeineh Scanning Video Densitometry system.
Data acquisition and analysis: HP 9000 series systems, running Consort30, LYSYS II, and Paint-A-Gate Plus (automatic 3-color analysis with spreadsheet-compatible output); MDADS and EASY2 systems; several MS-DOS systems offering MODFIT and ISOCONTOUR (Verity), Elite (Coulter), MultiCycle (Phoenix), along with other graphics and statistics programs (CorelDraw, Picture Publisher, Sigma PLOT, InSTAT QuattroPRO, and Excel).
3. Specialization Areas: Extensive experience in measurement of functional parameters of drug resistance via dye/drug efflux assay in both solid and hematopoietic tissues. Modulation of efflux-related resistance with efflux blocking agents. Immunofluorescent determination of p-glycoprotein expression (C219, MRK16). Measurement of other mechanisms of resistance; DNA topoisomerase II, bc1-2, and MDR1 expression; glutathione levels. Immunofluorescence and DNA content analysis expertise.
4. Special Opportunities: None at this time.
5. Special Courses Offered: Drug efflux modulation studies; FACScan instrument operation; DNA content analysis; procedures for labeling p-glycoprotein by immunofluorescence and immunohistochemistry.
6. Graduate Program: None at this time.
7. Local Accommodations: Hotel St. Regis is within 2 miles.
8. Transportation: Local: Detroit City Airport. Major airport: Detroit Metropolitan International.
9. Cost of Internship: $500.00 per week (negotiable).
10. Communication:
Awtar Krishan, Ph.D., Scientific Director Michigan Cancer Foundation 110 East Warren Detroit, MI 48201 Phone: (313) 833-0715 x-306 FAX: (313) 832-0531 E-mail: awtark@mcf.roc.wayne.edu
