Re: HP data conversion

Eric Martz (emartz@microbio.umass.edu)
Mon, 16 May 1994 13:03:03 -0500 (EST)

Below is a modified excerpt from a manuscript (submitted to Cytometry;
rejected; being revised) by Eric Martz entitled "MFI: a flow cytometry list
mode data analysis program optimized for batch processing under MS-DOS".
MFI is available by anonymous ftp from flowcyt.bio.umass.edu
/pub/flowcyt/mfi. The ASCII document file MFIINTRO.DOC available there is
the overview of MFI capabilities which is part of MFI's on-line help
system. MFITUTOR.EXE contains a tutorial with sample data.

TRANSFER OF DATA FILES FROM HEWLETT-PACKARD TO MS-DOS PC's.

At least four methods are available for transferring list mode data files
from Hewlett-Packard computers, which acquire the data from many flow
cytometers, to MS-DOS computers.

(i) Data written to three and one-half inch diskettes can be read on DOS
computers using commercial software. This method is too slow and cumbersome
for routine use, and the software is expensive.

(ii) Data can be transferred using serial ports and public domain
software. This is also very slow; several dozen data files take hours to
transfer; but the software is free (and available from the same anonymous
ftp site as MFI, /pub/flowcyt/hp2pc).

(iii) Data can be transferred from the IEEE-488 GPIB of the Hewlett
Packard (the thick cable which connects each subunit of the H-P computer)
to an IEEE-488 card installed in the PC bus. HPPCLINK programs for
managing the transfer were written at Becton-Dickinson and contributed to
the public domain. This method requires about 20 sec to transfer a 50K
file. Our card model GPIB-PC2 came from National Instruments (12109
Technology Blvd., Austin TX 78727). Further information generously
provided by David Coder is available by anonymous ftp from
flowcyt.cyto.purdue.edu in a file HPPCLINK.PS (or, if you don't have a
postscript printer, HPPCLINK.TXT) in /pub. The software is free, but
the PC card can set you back up to US$500.

(iv) If your Hewlett-Packard computer is equipped with a network interface,
data files can be transferred by FTP. This is the fastest method. Once the
data files are on an MS-DOS computer, they can be moved to other MS-DOS
computers efficiently by network FTP, or less efficiently by floppy
diskettes, backup tapes, etc.

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Dept Microbiology Voice: 413-545-2325 FAX: 413-545-1578
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