Thiazole Orange
DIETRICH@VT8200.DNET.LSU.EDU
Wed, 18 Jan 1995 14:49:01 -0600 (CST)
I am currently working with the dye thiazole orange, and so far the research
project has been limited to a FACScan. However, we'd like to move on to the
next step, which would involve sorting on a B-D FACS440. I have found a few
articles in the literature where individuals have used this dye on a FACSTAR
PLUS. Has anyone ever used this dye on a sorter? I thought it was in the same
chemical family as acridine orange, and I know that acridine orange gets
embedded in the tygon tubing and results in tubing replacement. The FACS440
has even more fluidic tubing than the FACSTAR PLUS. I was planning on just
running 10% bleach through the fluidics after the sort. Is this adequate for
removing all residual dye?
The working concentration we are using is 1 ug/ml. Thanks so much for any
comments on this topic.
Marilyn Dietrich
Louisiana State University
dietrich@vt8200.dnet.lsu.edu
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