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I response to Eric Martz comments:
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>Live rate displayed Rate saved to file Events lost
>on monitor to disk file
>3,200/sec 294 91%
>420/sec 357 15%
>
>Since the rate saved is actually slower at the higher rate, there is
>evidently a penalty for higher rates above and beyond the dead time
>per recorded event. I think everyone should do the kind of check you
>did. The conclusion (for our setup) is that concentrations of cells
>giving rates above 500/sec are simply wasting cells!
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According to a poster by Langlois and van den Engh presented at ISAC XVI,
March 1993: Both BD and MoFlo maximum data storage for 3-parameter list
mode was approx. 1000 events/sec.. A max.of 4000 events/sec. was observed
for "two-parameter histograms".
This should not be confused with the time required in signal processing
for sort-decision making - which is much faster. This is why I reported
trigger rates.
tom d.
"Never, for the sake of peace and quiet deny your own experience
or convictions"
Dag Hammarskjold
-- ============================================================================== Thomas Delohery | Internet: t-delohery@ski.mskcc.org Manager, Flow Cytometry Core Facility | Phone: (212) 639-8729 Memorial Sloan-Kettering Cancer Center | Fax: (212) 794-4019 ==============================================================================
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