FACSorting

Jonni Moore (moorej@a1.mscf.upenn.edu)
Wed, 03 May 95 10:34:01 -0400

We have had a FACSort for about a year and find it to be a great analytical
cytometer, however, we have yet to find a good sorting application. Is
anyone out there actually sorting with it? It would seem to be good for
small sorts of fairly abundant populations, but the dilution factor makes
recovery of small numbers a problem. B-D offers a "concentrator" to help
this problem (for $5K!!!), but does this actually work. The slow speed
makes it inappropriate for large sorts since viability then becomes an
issue. Any comments from those of you who are using the FACSort, and what
do you do with it?

Thanks!
Jonni Moore

Jonni S. Moore, Ph.D.
Director, Cancer Center Flow Cytometry and Cell Sorting Facility
University of Pennsylvania School of Medicine
297 John Morgan Bldg
Philadelphia, PA 19104-6082
Phone: 215-898-6853
FAX: 215-898-4227
e-mail: moorej@a1.mscf.upenn.edu

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CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu EMAIL robinson@flowcyt.cyto.purdue.edu