Dual Comp.

Thomas Delohery (t-delohery@ski.mskcc.org)
Tue, 12 Mar 1996 13:28:26 +0530

Hi all, the first time I saw PE showing up in the TRed I thought
either my optical filters were bad, my cables were switched
or the reagents were mislabelled. I couldn't believe PE
was absorbing out at 600nm. I then tried a CD3-PE from
BD that was even brighter in the TRed with a clear negative
population.

I was able to increase the wavelength out to around 605-610nm
with some loss of TRed signal. I think the only thing you can
do is select PE for the least abundant Ag and TRed for the most
abundant Ag.

So just how "reagent grade" are lots of PE (or APC)??

td

--
==============================================================================
 Thomas Delohery                        | Internet: t-delohery@ski.mskcc.org
 Manager, Flow Cytometry Core Facility  |    Phone: (212) 639-8729
 Memorial Sloan-Kettering Cancer Center |      Fax: (212) 794-4019
 1275 York Ave. Box 98                  |
 New York, NY    10021                  |
==============================================================================

Home Page Table of Contents Sponsors Web Sites
CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu EMAIL robinson@flowcyt.cyto.purdue.edu