PI dead cell gating/cell-cycle analysis

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I seem to recall a protocol for using propidium iodide for live/dead cell
gating and DNA staining for cell-cycle analysis of the same sample.

would anyone be able to send me a refernce for this or an outline of a
protocol?

If this procedure is not possible, could somebody recommend one which they use?

I will be using a FACScan for data accquisition.

Thank you very much for your help

Frank Isdell
The Rockefeller University
1230 York Ave
NY
NY 10021
Tel: 212-327-7657
Fax: 212-327-8875

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CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu EMAIL robinson@flowcyt.cyto.purdue.edu