Sub G1 Population References

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In recent months I have read from this space of the dangers of quantifying cell
death on the basis of the size of the sub G1 population. Concerns have been
expressed about a single cell yielding more than one fragment thus
overestimating the number of dead cells and such like.

I generally share these views but really need a suitable reference that
describes or discusses the difficulties of measuring cell death by this method.
All suggestions will be welcomed.

Thanks in anticipation.

Justin Lamb
Cell Pathology Lab
Aberdeen University Medical School
Scotland
j.lamb@abdn.ac.uk

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CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu EMAIL robinson@flowcyt.cyto.purdue.edu