Friday night flow special
Maryalice Stetler-Stevenson (stetler@box-s.nih.gov)
Tue, 14 May 1996 17:12:06 -0400
Does anyone have experience with leaving paraformaldehyde fixed stained
bone marrow cells for more than 18 hours? We got a specimen late on friday
from a patient with small non-cleaved lymphoma treated aggressively, now
for post treatment evaluation. We stained and fixed with 1%
paraformaldehyde. Unfortunately, due to a number of problems, acquisition
could not be performed the following day. In fact, acquisition was delayed
until Monday morning. The scatter looks good but there are basically no
B-cells (89% T-cells, 10% NK cells in lymphoid CD45/SSC gate). Previous
specimens also had a profound B-cell lymphopenia. Does anyone know if
B-cells are lost with time in paraformaldehyde?
Thanks for any help you can give.
Maryalice
Maryalice Stetler-Stevenson
Director Flow Cytometry Unit
Laboratory of Pathology, NCI, NIH
CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories
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