RE: Hoechst 33342 emission in apoptotic cells

Ross Longley (Longley@HBOI.EDU)
Fri, 26 May 95 13:58:00 PDT

Would be curious to know what you are using to induce your Jurkats to
apopotose? We have tried a number of things, including dexamethasone, to
try and get these things to do it--no luck--We are using P.I. to measure
apoptosis. Interestingly, PHA at 4.0 ug/ml induces Jurkats to apoptose.

Ross Longley, Ph.D.
Immunology Group Leader
Harbor Branch Oceanographic Inst., Inc.

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From: owner-cyto-sendout
To: Cytometry Mailing List
Subject: Hoechst 33342 emission in apoptotic cells
Date: Thursday, May 25, 1995 7:01PM

Hi all! Can anybody help us?

We are trying to detect apoptosis in Jurkat cells, with Ho33342.
We see a shift in Hoechst emission to longer wavelenghths in apoptotic cells

respect to non-apoptotic ones. Can anybody explain?

Thanks,

Jaume Comas
Unitat de Citometria de Flux
Serveis Cientifico-Tecnics.
Universitat de Barcelona, Spain.
E-mail jaume@giga.sct.ub.es

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