flourescent signal shifts
Jason, Janine (jmj1@CIDHIV7.EM.CDC.GOV)
Fri, 09 Jun 95 15:46:00 EST
To people doing intracellular (directly conjugated) and surface
staining(also directly conjugated):
Do you find you have flourescent signal shifts doing the above, vs doing all
surface staining (3-color)? If so, do you have any idea why it happens? If
not, could you share with me your protocol for intracellular staining?
Many thanks,
Janine Jason
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