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> I intend to use fluorogenic beta-galactosidase substrate to study gene
> expression in SW480-cells that are stable transfected with the gene encoding
> beta-gal (reporter-gene).....
We have been using the FluoReporter kit from Molecular Probes part
number F-1930 (50 test kit). We find it works pretty well. No fixation is
required and the cells can easily be sorted and even grown. Transfected
cells are usually about a log up from the control or untransfected cells.
You must however keep the sample cold as untransfected cells quite rapidly
convert the substrate at room temperature. We havn't co-labelled with
antibodies but I don't see why this should be a problem.
Good luck
Simon Monard
Cambridge
UK
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