TUNEL-mouse hepatocytes
Ton.Kiki (ton@NIEHS.NIH.GOV)
Fri, 14 Jul 1995 09:52:27 -0400
We're trying to detect apoptosis in treated mouse livers. The livers were
perfused with collagenase and teased apart to obtain cells. Hepatocytes were
fixed (2% paraformaldehyde), permeabilized (70% ETOH), and labeled by TUNEL.
Unfortunately, our results have been inconsistent and not very good lately
(no labeling). We use TdT and fluorescein-dUTP from Boehringer Manheim.
Is anyone doing something similar and/or have helpful suggestions? Any help
would be greatly appreciated.
K. Ton
NIEHS, Lab. Experimental Pathology
ton@niehs.nih.gov
CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories
and distributed free of charge as an educational service to the cytometry community.
If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL,
Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu
EMAIL robinson@flowcyt.cyto.purdue.edu
