RE:sorting onto slides

g.mesander (g.mesander@med.RUG.NL)
Tue, 19 Sep 1995 09:21:18 +0100 (MET)

In answer to your question does the sticky stuff work on slides?
We also are trying this technique out and use Poly-L-Lysine (Sigma) to
coat the slides before sorting cells directly on glass slides and
after incubation we fix the cells direct with 1 % paraformaldehyde
(15 minutes) that works OK as long as morfology is not the most
important thing.(The cells will be a lot smaller). We did not try
CELL-Tak or SUPER FROST. I hope this helps and I am interested in any
other method that could be used.

Geert Mesander
University Hospital Groningen
The Netherlands
Tel: Country Code-50-614578
e-mail: g.mesander@med.rug.nl

------- Doorgestuurd bericht volgt -------

Datum verzending: Mon, 18 Sep 1995 13:23:39 -0500 (EST)
Van: Frederic Preffer <preffer@helix.mgh.harvard.edu>
Onderwerp: sorting onto slides
Aan: Cytometry Mailing List <cytometry@flowcyt.cyto.purdue.edu>

what is your preferred technique for keeping cells stuck to glass slides,
post sorting, so as to permit subsequent fixing and morphological analysis
by cytochemistry? Any technique that works from sorted droplet DIRECTLY to
slide (that gets rid of saline), for those situations where there are too
few sorted cells to reanalyze/ make cytosmear with?
Does CELL-TAk , SUPER FROST , or some other sticky stuff work?
thanks.
`````````````````````````````````````````````````
Frederic I. Preffer
preffer@helix.mgh.harvard.edu
Department of Pathology- Cox5
100 Blossom St
Massachusetts General Hospital
Boston MA 02114
(617) 726-7481 {fax x2365}
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