Detection of Cy5
colette charland (ccharlan@moose.uvm.edu)
Thu, 2 Nov 1995 12:55:13 -0500
Greetings from the U. of Vt. I've just received a call from an investigator
who is going to label platelets with a FITC conjugated marker and an
activation marker linked to Cy5. They are using a kit from Amersham LIFE
SCIENCE called FluoroLink-Ab Cy5 Labeling Kit.The detection of plts. is no
problem, but I've never worked with Cy5. Does anyone have experience with
this kit and/or running such specimens on the flow. Any problems that I
need to know of in advance, i.e. quenching, compensation, coating of sample
tubing with the dye--you know the usual problems. Suggestions regarding
filter set up or any other input you have will be appreciated
Thanks in advance,
Colette Charland
ccharlan@moose.uvm.edu
CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories
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If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL,
Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu
EMAIL robinson@flowcyt.cyto.purdue.edu
