STAINING FIXED PLATELETS

gumbsc@CENTOCOR.COM
Tue, 28 May 1996 11:17:44 -0400

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Has anyone had success in staining fixed platelets with a p-selectin marker (eg. S12-FITC
or CD62)? Becton Dickinson claims that it can be done using 1% paraformaldehyde
fixation. I have followed their protocol for activation (substituting PMA for ADP) and
fixation. I have been able to stain fixed platelets with a pan-platelet marker (10E5 and
CD42a) but not the p-selectin markers. (All markers perform successfully on unfixed
platelets.) Any insights?

Cindy Gumbs
Assoc. Scientist II
Centocor, Inc.
200 Great Valley Pkwy
Malvern, PA 19380
(610) 696-7668
FAX (610) 651-6201

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CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu EMAIL robinson@flowcyt.cyto.purdue.edu