Re: retic analysis with high WBC

Bruce Davis, MD - Clinical Pathology (bdavis@beaumont.edu)
Thu, 13 Jun 1996 05:05:52 -0700 (PDT)

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In reply to: Dahl-Chase Pathology: "retic analysis with high WBC"

Andrea - I would like to modify your statement regarding WBC interference
with FCM retic analysis withg thiazole orange. WBCs can potentially
interfere with the retic % and immature reticulocyte fraction (IRF) only
if one does not gate out the WBC based upon there higher TO fluorescence
(approx 5-10X that of retics). See Cytometry 14:318-26, 1993, Heme/Onc
Clinics of N Amer 8:617, 1994, cytometry (CCC) 22:35-39, 1995, or
Laboratroy Hematology 2:2-8, 1996 for reviews. I highly recommend Verity
Software's ReticFit program, which simplifies FCM retic data analysis
from any instrument with regard to both elimination of WBCs and IRF
measurements. Call or email me directly if you have any other specific
questions.

Bruce H. Davis, M.D.
****************************
Director of Analytical Cytometry
Dept. of Clinical Pathology
William Beaumont Hospital
3601 W. 13 Mile Rd.
Royal Oak, Michigan 48073-6769
810-551-5137
FAX 810-551-8057

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In reply to: Dahl-Chase Pathology: "retic analysis with high WBC"

CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu EMAIL robinson@flowcyt.cyto.purdue.edu