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We did a pilot study recently on five "normal" lab volunteers to determine the
feasibility of developing ranges for using individuals as fresh normal
controls. Each person was sampled between 11 and 15 times over the course of
six months, and each specimen was collected between 7:30 am - 9:00 am to reduce
the effects of diurnal variation. Instrument setup, staining and gating were
in accordance with CDC guidelines. For these five individuals, the mean
CD3+/CD4+ percentages (1 SD) were: 44.7 (2.8), 49.6 (1.9), 46.5 (1.5), 34.3
(1.6), and 46.0 (2.7). Using a +/- 2 SD range, the expected variability in any
particular individual appears to be on the order of 6-11%.
I hope this is useful.
Bill Ward
Immunology & Molecular Diagnostics Branch
Department of Pathology
Wilford Hall Medical Center
>Date: Fri, 28 Jun 1996 16:00:48 -0400
>To: Cytometry Mailing List <cytometry@flowcyt.cyto.purdue.edu>
>From: mek4@psu.edu (Elaine Kunze)
>Subject: CD4 (daily variations)
>
>
>Can anyone tell me how CD4 values vary from day to day? (ie. one human,
>tested everyday for a week, for a month, etc.) Is there a reference
somewhere?
>
> ************************************
> Elaine Kunze
> Flow Cytometry......Image Analysis
> 8B Althouse Lab
> (814) 863-2762
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