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2. S phase interpretation is more difficult on paraffin due to
greater quantities of debris from cut nuclei.
3. Quality of histograms is very much linked to fixative
employed: as a rule the more acid the fixative the worse the histogram.
This may be important when using tissue sourced from different labs.
4. External reference standards are not possible when using praffin
embedded tissue. This is particularly important in diploid samples.
Eric P Miller
Edinburgh Medical Oncology Unit
"Don't anthropomorphise computers. They hate that."
On Mon, 8 Jul 1996, Mazharov wrote:
>
> Hello everyone!
>
> I would like to know an up-to-date opinion about DNA ploidy analysis of
> paraffin-embedded breast tissue vs fresh breast tissue.
>
> Is it good idea to switch from running fresh breast tissue to
> paraffin-embedded breast tissue?
>
> Any reply will be appreciated.
>
> Mike
>
>
>
>
>
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