Reply: Hybridoma against CD3
RCherv@mail-sh.lsumc.edu
Sun, 28 Jul 96 11:17:54 cst
Petter,
We have used the YCD3 antibody for surface (and intracellular)
staining of mouse cells with great success. This is a rat anti-mouse
CD3 epsilon antibody, originally developed by Dr. Kim Bottomly.
Various conjugated forms are available from Gibco BRL, Grand Island,
NY. We have also obtained the 29B hybridoma producing the antibody
from Dr. Bottomly. She is very generous in making it available and I
doubt you would have any problem obtaining it from her, should you
need to. Her address is: Dr. Kim Bottomly, Professor, Dept. of
Immunobiology, Yale University School of Medicine, 310 Cedar St., New
Haven, CT, USA, 06520-8011. I hope this helps.
Best Wishes,
Robert Chervenak, Ph.D.
Director,
LSU Core Facility for Flow Cytometry
CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories
and distributed free of charge as an educational service to the cytometry community.
If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL,
Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu
EMAIL robinson@flowcyt.cyto.purdue.edu
