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>I am trying to track down a supplier for 2',7'-Dichlorofluorescin Diacetate or
>2',7'-Diacetyl Dichlorofluorescin for an oxidatice burst assay.
>
>The reference I have is Bass 1983, J Immunol and that states Eastman Kodak
>as a supplier but als they do not have it, I have tried ICN, Sigma, can
>anyone help me please.
>
>Cheers
>Aaron Rae
>Flow Cytometry Technician
>North Queensland Clinical School
>University of Queensland
>
>Phone: 61 77 819660
>Fax: 61 77 819649
The most sensitive flow cytometric indicator of oxidative burst
activity is not dichlorofluorescin (DCF), but dihydrorhodamine 123 (DHR).
While DHR has other uses in flow, it is ideal for detection of oxygen
reactive species, and is 7 fold brighter than DCF under the same
conditions. The DHR assay can be performed using al little as 200ul of
whole blood, involves a technically simple procedure, and unambiguously
indicates NADPH oxidase activity.
Here is the reference.
Flow Cytometric Analysis of the Granulocyte Respiratory Burst: A
Comparison Study of Fluorescent Probes.
Journal of Immunological Methods 178 (1995) 89-97
DHR is available through Molecular Probes in Eugene OR. (catalog #
D-632)
DCF is no longer available through Eastman Kodak. If you still
want it, try Fisher Scientific (1-800-766-7000), catalog # AC40688-0010.
Sarah Vowells
NIAID, NIH
svowells@nih.gov
(301) 496-4879
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