protocols for cho cells

Martin, Jill V. (martin.jill@mayo.edu)
Fri, 23 Aug 1996 11:26:33 +0000

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We are studying the expression of a protein with several putative membrane
spanning domains using synthetic peptide antibodies. We are interested in
hearing from individuals with experience in FACS analysis of substrate
attached CHO cells, including methods of detachment, suspension,
permeabilization and fixation suitable for FACS analysis.

You may reply to Jill Martin as above, or to me directly:
mcdonald.john@mayo.edu.

Thanks in advance, John McDonald, Mayo Clinic Scottsdale.

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CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu EMAIL robinson@flowcyt.cyto.purdue.edu