BrdU Staining

ronald hill (ronald@sugen.sf.ca.us)
Tue, 27 Aug 1996 18:22:33 -0700

Hi,
We are trying to use BrdU staining to identify cycling cells in 32D
cultures (murine myeloid precursors). Control asynchronous cells in
log phase showed no positive cells with anti-BrdU. PI stain showed
plenty of S-phase cells. Control 3T3 cells stained as expected with
anti-BrdU. We use 10 micromolar BrdU for 1 hr before fixing. Is there
something unusual about 32D cells with respect to this protocol? Any
ideas would be appreciated.

Ron Hill, Ph.D.
Research Scientist
Sugen, Inc.
Redwood City, CA
ronald@sugen.sf.ca.us
tel 415-306-7797
fax 415-369-0679

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