Flow Sorting of Neurons...

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I'm interested to sort preparations of neurons isolated from mousebrain on
a FACStar Plus (no Turbo or Macrosort option). Does anybody have experience
with this. Basically my problems are the following:

1. Is there a good cell isolation procedure of the cells from fresh brain
tissue, that does not kill them, but results in round (no axons) neurons
that one could run through the sorter (I know one with trypsin + DNAse, but
like to hear if there are other smart ones out there....).

2. Is there any other differences in the sort setup (do you need a bigger
nozzle, or Macro Sort option ?) .

Thanks for any hints on the subject.

Matthias

______________________________________________________________
Matthias Haury _/_/_/ _/_/_/ _/_/_/
Flowcytometry - Immunology _/ _/ _/ _/ _/
Institut Pasteur Paris _/ _/_/_/ _/_/_/
Email: mhaury@pasteur.fr _/ _/ _/
Tel: 33 (1) 40 61 31 29 _/_/_/ _/ _/
Fax: 33 (1) 45 68 86 39 INSTITUT PASTEUR PARIS
______________________________________________________________

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CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu EMAIL robinson@flowcyt.cyto.purdue.edu