Re: Distance between sort streams
Jim Houston (jim.houston@stjude.org)
Fri, 13 Sep 1996 08:10 -0500 (CDT)
The angle of the sort streams are determined by two votages. One on
the plates which is non adjustable and the other on the sorted drops
which are. If the streams are not out far enough I would check the
eletrical connections to the flow head for tightness, Check that the
connections for the plates are snug and clean, also check the charge
wire which is on the flow head holder that touches the sample input
port on the head. A problem in any of these will decrease voltage
to your drops.
Less sheath pressure will increase the distance.
I usually set the distance at the top of the plates using a cotton swab
by inserting it between the plates. I have not noticed an increase in
stream deflection by changing the angle of the plates.
If you cannot reslove the problem then you will need someone to check the
voltages delivered to both the plates and drops.
Good Luck
Jim Houston
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