Re: Flow on Human Hepatocytes
Andreas Schrer (h82@ix.urz.uni-heidelberg.de)
Fri, 13 Sep 96 21:24:31 +0100
>A colleague has asked me if I would help him with his human hepatocyte
>cultures.
>
>Is anyone aware of an antibody to hepatocyte surface protein(s) which
>would allow me to use flow to detect the percentage of hepatocytes in the
>cultures? I have found anti-HGF in DAKO's catalog but am not sure if the
>HGF receptor is expressed on these cells in culture.
Well, I don't know about the HGF receptor.
The asialoglycoprotein receptor is a specific marker for hepatocytes =
(HC), but
it is - as far as I know - not commercially available yet and it's =
expression
is downregulated in culture within a few days. You might also try to
discriminate HC from non parenchymal cells by antibodies to cyto-
keratin: HC stain Cytokeratin 18 pos. and Cytokeratin 19 neg. =
(bileduct
epithelia are both Cytokeratin 18 and 19 pos.).
--
Dr. Andreas Schr=F6der, MD
Inst. of Immunology
Univ. of Heidelberg, Germany
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