Re: PI and Yeast
RCherv@mail-sh.lsumc.edu
Fri, 20 Sep 96 16:42:02 cst
Folks-
We have an investigator here who is looking at cell cycle in yeast
using P.I. We've notice what to us is a weird phenomenon: When the
cells are grown at 37 C, the staining looks pretty good (at least for
yeast). However, when the cells are grown at lower temps, such as 30
C or 11 C, the peaks get very broad, and the staining intensity
increases dramatically. Note that the cells are stained under
identical conditions, they have only been grown at different temps.
How could the growth conditions affect the binding of P.I. to DNA
(note, too, that the samples were all treated with RNase)? Is there a
temperature-dependent change in the conformation of the DNA that
alters P.I. binding? Does temperature alter particular DNA-protein
interactions that affect P.I. binding? Any suggestions, thoughts,
speculations or just plain guesses would be most helpful.
Thanks tons,
Rob Chervenak, Ph.D.
Director,
LSU Medical Center Core Facility for Flow Cytometry
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