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Male Germ Cell Studies
Analytical Cytology Facility
Division of Environmental Toxicology
Department of Environment
ENEA CRE Casaccia
00060 Roma, ITALIA
Laboratory Profile
Director: Dr. Marcello Spano
1.Facilities available: 150 m 2 facility containing instrumentation lab, general biology lab, computer facilities, tissue culture facility, office space for visiting scientists. Animal rooms are in a separate space.
2. Instrumentation:
Flow cytometry: Becton Dickinson FACStar flow cytometer / sorter with Spectraphysics 2W argon laser; Partec Pas III flow cytometer. Also available BD FACStar PLUS dual laser (5W Ar/UV + 2W Ar/Vis) flow cytometer.
Other major items: Fluorescent microscope with a large variety of optical filters (double and triple-bandpass included); BioRad MRC 600 confocal microscope with air cooled argon laser.
Computers and Analytical: Standard software on the flow cytometer dedicated computers; LYSIS II also available; Original softwares for DNA content analysis and general flow cytometry data management/analysis running on our IBM-PC based (DOS and OS2) workst ation; Phoenix MultiCycle and Listview. Non cytometry softwares for statistical analysis, graphics and word processing on IBM and Maclntosh.
3. Specialization areas: DNA content/structure analysis on testis cells and sperm (human / mouse). Physiological studies (viability, mitochondrial activity and mass). Specialty usage of several DNA specific fluorescent probes. Our lab has extensive ex perience in cell kinetic studies; flow cytometric immunophenotyping applied to mouse immunology studies; FISH techniques for genetic toxicology applications.
4. Training Opportunities: Isolation, preparation, handling and usage of mouse and human germ cells. Several monoclonal antibodies available for training purposes in immunophenotyping.
5. Special courses offered: FACStar and Partec instrument usage; DNA staining and analysis; immunophenotyping; Facilities available for training on several computer analysis packages.
6.Graduate Program: Program for doctoral thesis offered to university students in several areas using cytometric techniques related to studies in genetic toxicology.
7.Local Accomodations: Several economic hotels are available in Anguillara ( 5 km from the research center), a small town on the volcanic lake of Bracciano, a beautiful holiday resort. Many others, but generally more expensive (about 70 $) are located in Rome near the ENEA headquarters.
8. Transportation: ENEA CRE Casaccia is the largest multidisciptinar technological center in Italy, It is located 25 km north-west from the Rome center. Company shuttles are available free of charge from/to ENEA headquarters downtown and, in the mornin g and in the afternoon, company bus lines commute employees from/to the research center with many urban areas. Public bus lines (Rome-Bracciano/Angullara) and trains (Cesano-Rome) are also available (about 3$). Closest major airport is Rome Fiumicino ( F CO),
9. Cost of Internships: Depends upon what studies are required. A base rate of 500 $ per week will cover most laboratory costs including basic reagents and up to 10 hours of flow cytometry instrument time, The best time for internship is during late spr ing-early summer, always by arrangement.
Dr. Marcello Spano
Director, Division of Environmental Toxicology
ENEA CRE Casaccia
00060 Roma, ITALIA
Phone: (39) 6 3048 4737
FAX: (39) 6 3048 6559
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