Great Lakes International Imaging and Flow Cytometry Association
GLIIFCA
Program for the 1995 GLIIFCA Meeting, Detroit, MI, Sept 30-Oct 1, 1995
Fourth Annual Meeting
Great Lakes International Imaging and Flow Cytometry Association
September 29, 30 - October 1, 1995
St. Regis Hotel, Detroit, Michigan
Corporate Members and Meeting Sponsors for the 1995 Meeting
Becton Dickinson Immunocytometry Systems
Bio Rad, Inc.
CalTag, Inc.
CompuCyte Corp.
Coulter Corporation
Coulter Electronics of Canada, Ltd.
Cytomation, Inc.
DAKO Corporation
Flow Cytometry Standards Corporation
Harlan Bioproducts for Science
Immunotech, Inc.
Medical Science Associates
Miltenyi Biotec Inc.
Ortho Diagnostics Systems, Inc.
PharMingen
Phoenix Flow Systems, Inc.
R&D Systems, Inc.
Sigma Immunochemicals
Spherotech, Inc.
Verity Software House, Inc.
and
ROSWELL PARK CANCER INSTITUTE
BUFFALO, NEW YORK
Steering Committee
David Hedley - Ontario, Canada
Charles Goolsby - Illinois
Maurice R. G. O-Gorman - Illinois
J. Paul Robinson - Indiana
Alexander Nakeff- Michigan
Waclaw Jaszcz - Minnesota
Linda Setterlund - Minnesota
John M. Lehman - New York
Carleton C. Stewart - New York
Charles L. Hitchcock - Ohio
James W. Jacobberger - Ohio
Betsy Ohlsson-Wilhelm - Pennsylvania
Lynn Peterson - Wisconsin
J. Paul Robinson & Julie Auger, Program and Steering Committee Chairs 1995
Alexander Nakeff, Local Host
Friday Evening September 29
8:00 - 11:00 PM Reception, Open Bar 8 - 10 PM (Exhibit/ Poster Area)
Sponsored by PharMingen
Saturday Sept. 30
7:30 - 9:00 AM Continental Breakfast in Exhibit/ Poster Area
8:45 AM Welcoming Remarks
Plenary Session I -Cell Function by Flow Cytometry (Woodward Room)
Julie Auger, Paul Harris.... Chairs
9:00 - 9:50 David Hedley, Princess Margaret Hospital, Toronto, Ontario,
Chalk Board Review- "Mapping the Death Pathway with Live Cell Probes"
9:50 - 10:10 John Semple, St. Michael's Hospital, Toronto, Ontario -
"Analysis of Platelet Populations in Autoimmune Thrombocytopenia (AITP):
Elevated HLA-Dr Coexpressed with Macrophage and Red Blood Cell Molecules."
10:10 - 10:30 William Telford, University of Michigan Medical School, Ann
Arbor, Michigan- "Measurement of Plasma Membrane Ca++ ATPase Activity in
Inside-Out Membrane Vesicles by Flow Cytometry".
10:30 - 11:00 Coffee Break-- Exhibit/Poster Area - Sponsored by Caltag
11:00 - 11:20 Maurice O'Gorman, Children's Memorial Hospital, Chicago,
Illinois - "T-Cell Activation and Proliferation by Flow Cytometry."
11:20 - 11:40 Anne Sperling, University of Chicago, Chicago, Ilinois -
"T-Cell Costimulation - A Life or Death Situation."
11:40 - 12:00 Steven Rehbein, Roswell Park Cancer Institute, Buffalo, New
York - "Evaluation of Cytokine Receptor Expression on Normal Human
Hematopoietic Progenitor Cells Using 4-color Multiparameter Flow
Cytometry."
12:00 - 1:00 Box Lunch in Commons Room (Steering Committee Mtg.)
1:00 - 2:00 Poster Viewing (Exhibit/ Poster Area)
Plenary Session II - Image Cytometry (Woodward Room)
T. Vincent Shankey and J. Paul Robinson... Chairs
2:00 - 2:50 J.Paul Robinson, "Introduction to Confocal Microscopy"
3:00 - 3:20 Stan Shackney, Allegheny General Hospital, Pittsburgh, PA,
"Clinical Applications of Tumor Biology: Imaging and Flow Methods Compared"
3:20 - 3:40 Vince Shankey, Loyola Univ. Med. Ctr. Maywood, IL,
"Technical and Biological Implications of Image Analysis of Clinical
Specimens"
3:40 - 5:00 Posters/ Exhibits Wine and Cheese Happy Hour- Sponsored
by Compucyte Corporation
Presidential Symposium: (Woodward Room)
Presidential award selected from abstracts
5:00 - 5:15 (Tech Award) Avis Peters, United Hospital, St. Paul,
Minnesota. "Optimization of Viability, Lineage, and Light Scatter
Parameters for Multiparameter Flow Analysis of Bone Marrow Samples."
Selected Presentations Not Competing for Awards:
5:20 - 5:40 Michael Sramkoski, Case Western Res. Univ., Cleveland, OH,
" Simultaneous Determination of Cyclin B1, P105 and Cell Cycle in DU145
Cells"
5:40 - 6:00 Karen Cornell, Purdue University, West Lafayette, IN, "Flow
Cytometric and Image Analysis of Aminopeptidase Activity Within Neoplastic
Cells."
6:00 - 6:10 Lori Anderson, Victoria Hospital, London, Ontario, "Quality
Assurance for Stem Cell Enumeration in Canada"
Business Meeting
6:10 - 6:30 Presentations: Technician Award and Travel Awards
Sponsored by: BioRad, Caltag, Compucyte, Coulter, DAKO, FCSC,
PharMingen, Spherotech, and Verity Software House
6:30 - 8:00 Free Time (Bar available in Commons Room)
8:00 - 12:00 FLOW-O-WEEN - FLOWDOWN (Woodward Room)
SPONSORED BY: BECTON DICKINSON IMMUNOCYTOMETRY SYSTEMS
Sunday Oct 1
7:30 - 8:30 AM Continental Breakfast in Exhibit/ Poster Area
Plenary Session III -Developments In Instrumentation and
Applications(Woodward Room) Gary Durack and James Jacobberger... Chairs
8:30 - 8:55 Steve Kelley, Purdue University, West Layfayette, IN. "See
the World Famous MYSTERY SPOT!" - An abbreviated guide to Cytometric
Tourist Traps on the Information Superhypeway.
9:00 - 9:20 Douglas Berger, Compucyte Corp., Cambridge, MA, "Laser
Scanning Cytometry: Cellular Analysis by Fluorescence with Automated
Visualization of Restained Cells"
9:25 - 9:55 Gary Durack, University of Illinois, Urbana, IL,
"Fluorescence Lifetime Measurement via Cross Correlation Methods for Image
and Flow Systems"
10:00 - 10:25 James Jett, Los Alamos National Laboratory, Los Alamos, NM,
"Molecular Analysis by Flow Cytometry"
10:30 - 10:45 Coffee Break-- Exhibit/Poster Area- Sponsored by Coulter
10:45 - 11:10 Abe Schwartz, Flow Cytometry Standards Corp., San Juan, PR,
"How to Construct a Standardized Calibration Curve"
11:15 - 11:40 Phyllis S. Frisa, Case Western University, Case Western
Res. Univ., Cleveland, OH, "On the Transformation of Fluorescence Cytometry
into Molecular Quantities"
11:45 - 12:10 Andrew Yen, Cornell University, Ithaca, NY, "Testing
Transregulatory Activity of the RB Tumor Supression Gene During the Cell
Cycle with Multiparameter Flow Cytometry: RB, MYC, FOS, and DNA"
12:15 - Closing comments and adjournment of meeting -Carleton Stewart
N.B. A brunch (pay your own) will be available in the restaurant after 12 PM.
NEW INFORMATION ...... Cytometry
Meetings in the next months..
For information about this page contact
J.Paul Robinson, Ph.D, Director PUCL, 1515 Hansen B050, Purdue University, West Lafayette, IN 47907-1515, U.S.A.
Phone: (317) 494-0757; Fax: (317) 494-0517: Send an email request
now to J. Paul Robinson:
robinson@flowcyt.cyto.purdue.edu
CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories
and distributed free of charge as an educational service to the cytometry community.
If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL,
Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu
EMAIL robinson@flowcyt.cyto.purdue.edu
