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The abstract are printed in the: Proceedings of the DGZ Heidelberg Meetings, DKFZ, Heidelberg 1995, ISSN 0949-5347
=== ORAL PRESENTATIONS ===
1. CELL KINETIC EFFECTS INDUCED BY INTERFERON GAMMA AND TUMOR
NECROSIS FACTOR ALPHA IN HUMAN TUMOR CELLS, INVOLVEMENT OF
CYCLINS
H. Baisch
Institute of Biophysics and Radiobiology, University of
Hamburg, Martinistrasse 52, D-20246 Hamburg
2. ROLE OF PROTEIN KINASE ACTIVITY IN REGULATION OF CELL CYCLE
PROGRESSION
H. Crissmann, J. Valdez, S. Rose, S. Minter
Life Sciences Division Los Alamos National Laboratory, USA
3. STROMA ORIGINATING FROM SORTED CD13-POSITIVE BONE MARROW
CELLS
J.W. Ellwart, K. Nispel, R.A.J. Oostendorp, G. Ledderose*, P.
Dörmer
GSF-lnstitut für Experimentelle Hämatologie, München,
*Med. Klinik III, Klinikum Grosshadern, Universität München
4. CELL CYCLE KINETICS OF UROTHELIAL CELL LINES AS MEASURED BY
FLOW CYTOMETRY USING BRDU/HOECHST TECHNIQUES AND
IMMUNHISTOCHEMICAL STAINING FOR PROLIFERATION ASSOCIATED
ANTIGENS
E. Endl, G. Fauser, P. Steinbach, R. Knüchel and F.
Hofstädter
Institute of Pathology, Franz-Josef-StrauB Allee 11, D-93053
Regensburg
5. DETECTION OF MINIMAL RESIDUAL DISEASE IN ACUTE MYELOGENOUS
LEUKEMIA AND MYELODYSPLASTIC SYNDROMES IN CLINICAL REMISSION
BY FACS/FISH
H. Engel,1 A. Gooodacre, A. Keyhani, S. Jiang and M. Andreeff
Department of Hematology, University of Texas M. D. Anderson
Cancer Center, Houston, USA and1 Institiut für klinische
Biochemie der Universität Bonn, Germany
6. ANALYSIS OF PARTICLE-SIZE FOR TESTING ON CYTOTOXICITY
D. Fieblinger, K. Grünheid, D. Labes
Pharmakologische Forschungsgesellschaft BIOPHARM GmbH Berlin,
Alfred-Kowallke-Strasse 4,10315 Berlin
7. ANGULAR RESOLVED LIGHT SCATTERING OF SINGLE PARTICLES AND
PARTICLE AGGLOMERATES IN A FLOW CYTOMETER
C.Gohlke, J.Neukammer, and H.Rinneberg
Physikalisch-Technische Bundesanstalt, Berlin-Charlottenburg,
Abbestrasse 2-12, D-10587 Berlin,Germany
8. FLOW CYTOMETRIC S-PHASE DETERMINATION IS A STRONGER
PROGNOSTIC FACTOR IN NODE-NEGATIVE BREAST CANCER THAN
IMMMUNOCYTOCHEMICAL MIB1 (Ki-67) STAINING
N. Harbeck, P. Dettmar1, C. Thomssen, L. Pache, M. Schmitt, F.
Jänicke, W. Nathrath1, and H. Graeff
Frauenklinik and 1 Institut für Pathologie, Technische
Universität, D-81675 München, Germany.
9. MODEL SYSTEM FOR ISOLATION OF COMPETENT OVARIAN CARCINOMA
CELLS FROM FRESH TUMOR TISSUE BY A MAGNETIC SEPARATION SYSTEM
(MACS)
N. Harbeck, A. Abdulsalam, S. Schwarze, E. Schüren, P.
Dettmar1, W.Kuhn, H. Graeff, and M. Schmitt
Frauenklinik and 1 Institut für Pathologie, Technische Universität, M&u
uml;nchen, Germany.
10. INFLUENCE OF FLUORESCENT LABELING OF GLYCOSAMINOGLYCANS
(GAGs) FOR BINDING TO LEUKOCYTES
J. Harenberg. R. Malsch. L. Piazolo. G. Huhle. and D.L. Heene
1st Department of Medicine, Faculty for Clinical Medicine
Mannheim of the University Heidelberg, Theodor-Kutzer-Ufer,
D-68167 Mannheim
11. SERIAL FLOW CYTOMETRY DURING CHEMOTHERAPY: IMPACTS ON THE
DEVELOPMENT OF INDIVIDUALIZED TREATMENT STRATEGIES
J. Hemmer
University of Ulm, Germany, Division of Tumor Biology
12. DIFFERENT APPROACHES TO STUDY THE NUCLEOLAR TERRITORY
DURING THE CELL CYCLE
D. Hernandez-Verdun, C. Masson and R. Junera
Institut Jacques Monod, 2 place Jussieu, 75251 Paris Cedex
05, France
13. QUANTITATIVE ANALYSIS AND QUALITY ASSURANCE FOR FLOW
CYTOMETRY
H.-G. Höffkes und G. Schmidtke
Abteilung für Hämatologie, Zentrum fur Innere Medizin,
Universität Essen, Hufelandstrasse 55, 45122 Essen
14. ISOLATION AND CHARACTERIZATION OF PERIPHERAL BLOOD
DENDRITIC CELLS BY HIGH-GRADIENT MAGNETIC CELL SORTING
Alexander Horst*, Andreas Radbruch#, Stefan Miltenyi* and
Jürgen Schmitz*
* Miltenyi Biotec GmbH, Bergisch-Gladbach, Germany and
#Institute for Genetics, University of Cologne, Cologne,
Germany
15. COMBINATION OF B-CELL PURGING AND CD34+ SELECTION FOR
LYMPHOMA CELL REMOVAL FROM BLOOD STEM CELL AUTOGRAFTS
P. Hundsdörfer1, S. Frühauf2, R. Höft1, R. Haas2, W. J.
Zeller1
1 German Cancer Research Center, Research Program Diagnostics
and Experimental Therapy, Heidelberg, Germany, 2 Department
of Internal Medicine V, University of Heidelberg
16. A NEW 3-COLOUR LYSE-NO-WASH (LNW), ABSOLUTE COUNT SYSTEM
FOR IMMUNOPHENOTYPING AND HIV MONITORING
Luc Ketele
Becton Dickinson Immuncytometry Systems Europe, Erembodegem,
Belgium
17. "TERBIUM-NUCLEOTIDES" FOR LUMINESCENT LABELLING AND
SENSITIVE TIME-RESOLVED DETECTION OF NUCLEIC ACIDS
S. Klingel, M. Collasius and G. Valet
Arbeitsgnuppe Zellbiochemie, Max-Planck-lnstitut für
Biochemie, D-82152 Martinsried
18. IMPACTS OF THE DNA PLOIDY STATUS OF THE PRIMARY TUMOR
OKKULT METASTASIS IN ORAL SQUAMOUS CELL CARCINOMA
K. Kraft*, A Schouba#, J.Hemmer#
*Dept of Pathology, Military Hospital Ulm, Germany,# Division
of Tumor Biology. University of Ulm, Germany
19. DIFFERENTTIATION OF OVARIAN TUMORS WITH IMAGE ANALYSIS
W. Kuhn, M. Ruhnke, A. Ditzenbach, H. Weitzel
Department Gynecol. Obstet., Klinikum Benjamin Franklin Free
University of Berlin, Germany
20. NEW DEVELOPMENTS IN CONFOCAL LASER SCANNING MICROSCOPY
J.Kukulies
Carl Zeiss Jena GmbH, Unternehmensbereich Mikroskopie,
Systemvertrieb Deutschland
21. DETECTION OF MDR-1 mRNA BY RT-PCR AND FLOW CYTOMETRY
D. Lassner, J. Milde, M. Ladusch*, K. Drössler* and H.
Remke
Institute of Clinical Chemistry and Pathobiochemistry,
*Institute of Zoology, University Leipzig, Liebigstrasse 16,
D-04103 Leipzig
22. COMPARISON OF BrdU INCORPORATION AND PCNA AND Ki67
EXPRESSION IN HL-60 CELLS AFTER INDUCTION AND IRRADIATION
M. Lindinger*, A. Sendler**, K.-P. Gilbertz*, D. van
Beuningen*
*Institut für Radiobiologie, Akademie des Sanitäts- und
Gesundheitswesen der Bundeswehr, D-80901 München,
**Chirurgische Klinik und Poliklinik der TU München,
Klinikum "rechts der Isar", D-81644 München, Germany.
23. COMPARISON OF FLOW CYTOMETRIC METHODS FOR THE ANALYSIS
OF APOPTOTIC CELLS
Kristin Marx, Michael Nüsse
GSF-Forschungszentrum für Umwelt und Gcsundheit,
AG Durchflußzytometrie, D-85758 Oberschleißheim, Germany
24. FLOW SORTING OF PRINS LABELLED PLANT CHROMOSOMES
Armin Meister, Uta Pich and Ingo Schubert
Institute of Plant Genetics and Crop Plant Research,
D-06466 Gatersleben
25. PROGNOSTIC RELEVANCE OF PLOIDY AND S-PHASE IN RESECTED
CARCINOMA OF THE PANCREAS
H. Nekarda, J.D. Roder, K. Becker*, A. Rossmann, J.R.
Siewert
Department of Surgery and *Institut for Pathology,
Technische Universität München, Klinikum rechts der Isar
26. DEMONSTRATION OF THE NUMBER OF RESIDUAL LEUCOCYTES OR
PLATELETS IN FILTRATED RED BLOOD CELL CONCENTRATES AND
PLASMA PREPARATIONS
J. Neumüller, *D.W.M. Schwartz, *W.R. Mayr
Ludwig Boltzmann Institute for Rheumatology and Balneology, Vienna,
Austria *Blood Donation Center, Austrian Red Cross, Vienna,
Austria
27. ANALYSIS OF MICRONUCLEI BY FLOW SORTING AND FISH
M. Nüsse1, A. Slavotinek2, B.M. Miller3
1 GSF-AG Durchflußzytometrie, D-85758 Oberschleißheim
2 Dept. Clinical Genetics, Churchill Hospital, Oxford 3PRPT.
Preclinical Division, Hoffmann La-Roche AG, Basel
28. RESULTS OF THE COLLABORATIVE STUDY 1995 ON
STANDARDIZATION AND QUALITY ASSESSMENT OF DNA FLOW CYTOMETRY
Friedrich J. Otto
Fachklinik Hornheide, D-48157 Münster
29. CYTOMETRIC ANALYSIS AND ISOLATION OF TRANSFECTED CELLS
BY SURFACE EXPRESSED SELECTION MARKERS
K. Petry, R. Christine, G. Siebenkotten and A. Radbruch
Institute for Genetics ,University of Cologne, Weyertal 121,
D-50931 Cologne, Germany
30. SUBCELLULAR LOCALIZATION STUDIES WITH NOVEL
MITOCHONDRIAL STAINS
Martin Poot, Ananda G. Lugade, Jennifer Krämer, K. Sam
Wells, Laurie J. Jones, Lisa Gibson, Stephen T. Yue, David
Hanzel$, Victoria L. Singer and Richard P. Haugland
Molecular Probes, Inc., P.O. Box 22010 Eugene, OR 97402, and
$Molecular Dynamics, Sunnyvale, CA 94086, U.S.A.
31. CALIBRATING A FLOW CYTOMETER WITH FLUORESCENT LATEX
BEADS
Martin Poot, Sharon M. Swan, and K Sam Wells
Molecular Probes, Inc., Eugene, OR 97402, U.S.A.
32. EXTERNAL QUALITY CONTROL SURVEY OF IMMUNOPHENOTYPING OF
THE CENTRAL REFERENCE INSTITUTION IN GERMANY ISSUES OF
SPECIMENS AND INTERLABORATORY VARIABILITY
W. Prohaska, R. Kruse, W.-J. Geilenkeuser, T. Nebe, A.
Radbruch, H. Diem, A. von Rücker
Working group of quality assessment in flow cytometry of
the German Society of Clinical Chemistry
33. ANALYSIS AND ISOLATION OF RARE CELLS
Diether Recktenwald1, Hans Joachim Gross2, Stefan Miltenyi3
1 AmCell Corp., Sunnyvale, Ca, USA
2 Labor MittererstraBe, München
3 Miltenyi Biotec, Bergisch Gladbach
34. HIGH RESOLUTION FLOW CYTOMETRY AND P53 IMMUNOREACTIVITY
IN STAGE I BORDERLINE EPITHELIAL OVARIAN TUMORS
Reich O., Pürstner P., Schöll W.
Department of Obstetrics and Gynecology, University of
Graz, Auenbruggerplatz 14, 8010 Graz, Austria
35. QUANTIFICATION OF THE TOTAL CELLULAR CONTENT OF THE
MYELOMONOCYTIC PROTEINS MRP8 AND MRP14 AND OF THEIR
HETERODIMER RECOGNISED BY THE ANTIBODY 27E10 IN HUMAN
MONOSYTES AND GRANULOCYTES USING DUAL LABEL FLOW CYTOMETRY
O. Röhrig, C.L. Klein, T. van Kooten C.J. Kirkpatrick
Institute of Pathology, Universityhospital of Mainz,
Germany
36. CHARACTERIZATION OF PERIPHERAL BLOOD MONONUCLEAR
PHAGOCYTE SUBPOPULATIONS
G. Rothe, J. Stöhr, G. Schmitz
Institute for Clinical Chemistry and Laboratory Medicine,
University of Regensburg
37. A NEW FLOW CYTOMETRIC ASSAY FOR THE ANALYSIS OF THE
HUMAN LYSOSOMAL ACID LIPASE
G. Schindler, G. Rothe, G. Schmitz
Institute for Clinical Chemistry and Laboratory Medicine,
University of Regensburg, Germany
38. QUALITY CONTROL AND STANDARDIZATION OF FLOW CYTOMETRIC
PLATELET ANALYSIS
G. Schmitz, S. Barlage, G. Rothe
Institute for Clinical Chemistry and Laboratory Medicine,
University of Regensburg, F.R.G.
39. COMPARISON OF FLOWCYTOMETRIC DNA-MEASUREMENT WITH
MORPHOMETRIC IMAGE ANALYSIS PARAMETER IN SELECTED OVARIAN
CARCINOMA
Scholl W., Reich O., Pürstner P., Taucher A.
Department of Obstetrics and Gynecology, Univ. of Graz,
Austria
40. QUALITY CONTROL OF IMMUNEPHENOTYPING IN AUSTRIA
E. Schönwald*, R. Jilch**
** Central Laboratory ( Univ.Prof. Dr. M. Fischer ) and
* Department of Dermatology ( Univ. Prof. Dr. F. Gschnait )
Municipal Hospital Lainz, Vienna, Austria
41. SOURCES OF ERRORS IN QUANTITAVE IMAGE ACQUISITION IN
CYTOMETRY
Schwarzmann P., Mähner G.
Inst. für Physikallshe Elektronik, Univ. Stuttgart,FRG
42. FLOW CYTOMETRIC DETECTION OF INTRACELLULAR PLATELET
ANTIGENS
Peter Sedlmayr1, Barbara Grosshaupt1, 2, Wolfgang Muntean2,
Astrid Blaschitz3, Gottfried Dohr3
Departments of Internal Medicine1 and Pediatrics2, and
Institute for Histology and Embryology3,
Karl-Franzens-University Graz, Austria
43. CYTOMETRIC EVALUATION OF RUSH BEE-VENOM IMMUNOTHERAPY
J.A. Segura, J. Irsch*, A. Löhndorf*, N. Hünzelmann*
and A. Radbruch
Institute for Genetic. University of Cologne. Weyertal 121.
50931 Cologne, Germany.*Clinic of Dermatology of the
University of Cologne. Joseph-Stelzmann Str. 9.50931
Cologne, Germany.
44. 5-AMINOLEVULINIC ACID UPTAKE, SYNTHESIS OF
PROTOPORPHYRIN IX, AND EFFLUX OF PORPHYRINS IN TWO
UROTHELIAL CARCINOMA CELL LINES
P. Steinbach1, H. Weingandt1, R. Baumgartner2, M.
Kriegmair2, F. Hofstädter1, R. Knüchel1
Institute of Pathology, University of Regensburg and 2Dept.
of Urology, University of Munich, Germany
45. NON-RADIOACTIVE LIFE SPAN MEASUREMENTS OF RED BLOOD
CELLS BY FLOW CYTOMETRY AND ITS COMPARISON WITH RADIOACTIVE
LABELING
Trastl. C.1; Beisker. W.2; Berg D.3; Hoffmann-Fezer, G.1
(1): GSF - Institut fur Immunologie, (2): GSF -
Arbeitsgruppe Durchflusszytometrie, (3): GSF - Institut
für Strahlenbiologie, Ingolstädter Landstr. 1,D-85764
Oberschleissheim, Germany
46. SENSITIVITY OF MAMMALIAN SPERMATOGENIC CELLS TO
IONIZING IRRADIATION
I. Tatchen, W. Göhde
Insititut für Strahlenbiologie, Westfälische
Wilhelms-Universität, Münster, Germany
47. COMPUTER CLASSIFICATION OF FLOW CYTOMETRIC THREE COLOUR
IMMUNEPHENOTYPES FROM BLOOD LEUKOCYTES AND BONE MARROW OF
LOW AND HIGHGRADE NON-HODGKIN-LYMPHOMA AND CHRONIC
LYMPHATIC LEUKEMIA PATIENTS
G.Valet1, G.Schmidtke2, U.Schmücker2, G.Brittinger2,
H.G.Höffkes2
1) Max-Planck-lnst.f.Biochemie, 82152 Martinsried,
2) Abt.Hämatologie, Medizinische Univ.Klinik, 45122 Essen, Germany
48. EXCIMER FLUORESCENCE COMPARED TO DEPOLARIZATION IN THE
ANALYSIS OF HYDROPHOBIC MEMBRANE DOMAINS
M. Wimmer, G. Rothe, G. Schmitz
Institute for Clinical Chemistry and Laboratory Medicine,
University of Regensburg, Germany
=== Poster Presentations ===
49. CGH IMAGING BY A ONE CHIP TRUE COLOR CCD CAMERA
Harald Bornfleth1, Klaus Aldinger1, Michael Hausmann1, Anna
Jauch2 and Christoph Cremer1,3
1= Institute of Applied Physics, 2=lnstitute of Human
Genetics and Anthropology, 3=lnterdisciplinary Centre of
Scientific Computing (IWR), University of Heidelberg,
Heidelberg, F.R. Germany
50. SIMULTANEOUS REGISTRATION OF CALCIUM TRANSIENTS AND
REACTIVE OXYGEN INTERMEDIATES OF HUMAN POLYMORPHONUCLEAR
GRANULOCYTES IN RESPONSE TO ENVOIRONMENTAL AGENTS
W. Grundler1, P. Dirscherl2, K Marx1, I. Beck-Speier2,
W. Beisker1, K. Maier2, and A. Stampfl3
GSF-Forschungszentrum fiir Umwelt und Gesundheit,
AG Durchflußzytometrie, Institut für Inhalationsbiologie2
und Institut fur Toxikologie3, D-85758 Oberschleißheim
51. THE HEIDELBERG SLIT-SCAN FLOW FLUOROMETER
Michael Hausmann1, Alexander Rohrbach1, Dietmar Wolf1, Klaus
Aldinger1, Michael Schurwanz1, Jürgen Dölle2, Arnulf
Keese3, Martin Crone1, Christoph Cremer1
1= Institute of Applied Physics, University of Heidelberg,
D-69120 Heidelberg, Germany (present addresses: 2=lBM
Development Laboratory, D-71032 Böblingen, FRG; 3=
Bertelsmann Development, D-33311 Gütersloh)
52. FLOW-CYTOMETRIC CHARACTERIZATION OF BATCH CULTIVATED
BACTERIA WITH FLUORESCENT, rRNA-TARGETED OLOGONUCLEOTID
PROBES
Carsten Herrmann, Andreas Lösche, Thomas Bley
Projektgruppe Biosignale, Abteilung Biotechnologie,
Fakultät für Biowissenschaften,Pharmazie und Psychologie,
Universität Leipzig, Permoserstr. 15, 04138 Leipzig.
53. ANTI-P53 AUTOANTIBODIES IN SERA OF PATIENTS WITH ORAL
SQUAMOUS CELL CARCINOMA
Ch. Hofele*, M. Volkmann+, M. Schmitt*, W. Fiehn+,
J. Zöller*. J. Mühling*
(*Dept. of Oral and Maxillofacial Surgery, University of
Heidelberg, INF 400, D-69120 Heidelberg, Germany, +Central
Laboratory, Medical University Hospital, Bergheimer Str. 58,
D-69115 Heidelberg, Germany)
54. CELL CYCLE AND CELL SIZE ANALYSIS OF DIFFERENT
SACCAROMYCES YEASTS
K.-J. Hutter1, J.Schärfe2
1 DKFZ Heidelberg, Im Neuenheimer Feld 280, R.Süssmuth, Institut
f. Mikrobiologie, Univ. Hohenheim, 2 Schärfe System, Reutlingen
55. TIME SHIFTING OF CIRCADIAN PATTERNS OF LYMPHOCYTE
SUBSETS AND EXPRESSION OF CD2, CD11a AND CD44 IN HUMAN
PERIPHERAL BLOOD
T.O.Kleine1, R. Hackler1, P. Z&oouml;fel2, J. Albrecht3
1 Med. Zentrum für Nervenheilkunde, Funktionsbereich Neuro-
chemie, Universität Marburg a.d. Lahn, 2 Hochschulrechenzen-
trum der Universität Marburg, 3 Becton Dickinson, Heidelberg
56. INFLUENCE OF TOTAL SLEEP DEPRIVATION ON CIRCADIAN
VARIATIONS OF LYMPHOCYTE SUBSETS IN HUMAN PERIPHERAL BLOOD
FROM HEALTHY MALE VOLUNTEERS
T.O. Kleine1, W. Schreiber2, R. Hackler1, P. Zöfel2,
J.Albrecht3
1 Med. Zentrum für Nervenheilkunde, Funktionsbereich
Neurochemie, Universität Marburg a.d. Lahn; 2 Klinik für
Psychartrie im Med. Zentrum für Nervenheilkunde,
Universität Marburg a.d. Lahn; 2 Hochschulrechenzentrum der
Universität Marburg, 3 Becton Dickinson, Heidelberg.
57. DNA IMAGE CYTOMETRY ON SECTIONS: MEASUREMENT OF ENTIRE
OR CUT NUCLEI?
T.KIapperstück and W. Wohlrab
Department of Dermatology, Martin Luther University
Halle-Wittenberg, Germany
58. FAST KINETICS MEASUREMENT OF INTRACELLULAR Ca2+ ON THE
FACScan
Ladusch, M., Jüngel, A., Drössler, K.
University Leipzig, Institute of Zoology, Talstrasse 33,
D-04301 Leipzig
59. FLOWCYTOMETRIC TYPING OF PREADIPOCYTES IN MUSCLE TISSUE
Bertold Löhrke, Torsten Viergutz, Jochen Wegner, Klaus
Ender
Research Institute of Animal Biology, Dummerstorf-Rostock
60. FLOW CYTOMETRY ANALYSIS USING ACTIVATED MICROBEADS
Reinhard Malsch, Lukas Piazolo, Daniel Melui and Job
Harenberg
First Dept. of Medicine, Faculty of Clinical
Medicine Mannheim, University of Heidelberg, Theodor Kutzer
Ufer, 68167 Mannheim, Germany.
61. STANDARDIZATION OF PROTAMINE-COATED MICROBEADS USING
FLOW CYTOMETRY FOR HEPARIN QUANTITATION
Daniel Melui, Reinhard Malsch, Lukas Piazolo and Job
Harenberg
First Dept. of Medicine, Faculty of Clinical Medicine
Mannheim, University of Heidelberg, Theodor-Kutzer-Ufer,
68167 Mannheim, Germany
62. DETERMINATION OF BACTERIAL VIABILITY WITH A NOVEL 488
NM-EXCITABLE NUCLEIC ACID STAIN
Paul J. Millard, Bruce Roth, Stephen T. Yue, Laurie J.
Jones, and Martin Poot
Molecular Probes, Inc., Eugene,
Oregon 97402, U.S.A.
63. FLOW CYTOMETRIC DETECTION OF APOPTOSIS IN RAT T CELL
LINES
E.Mix1, U.K.Zettl1,2, J.Zielasek2, M.Stangel3,
K.V.Toyka2, H.-P.Hartung2, R.Gold2
1Dept. of Neurology, Hosp. Nerv. Dis.,Universität Rostock,
2Dept. of Neurology, Universität Würzburg,
3Dept. of Neurology, Freie Universität Berlin, Germany
64. DNA DISTRIBUTIONS OF SOME BACTERIA: INFLUENCE OF GROWTH
PHASE, GROWTH RATE AND NUTRIENTS
Susann Müller, Andreas Lösche and Thomas Bley
Projektgruppe Biosignale, Abteilung Biotechnologie,
Fakultät für Biowissenschaften, Pharmazie und
Psychologie, Universität Leipzig, Permoserstr.15, D-04318
Leipzig
65. FLOW CYTOMETRIC ANALYSIS OF THE PHYSICAL ASSOCIATION
BETWEEN SURFACE RECEPTOR AND CYTOSKELETON
B. Nebe, J. Rychly
Klinik für Innere Medizin, Universität Rostock
66. EVALUATION OF FOUR MONOCLONAL ANTIBODIES AGAINST
HLA-B27 FOR THEIR RELIABILITY IN HLA-B27 TYPING WITH FLOW
CYTOMETRY (FC). COMPARISON WITH THE CLASSIC
MICROLYMPHOCYTOTOXIC TEST (MLCT)
J. Neumüller, D.W.M. Schwartz**, E. Dauber**, W.R. Mayr**
Ludwig Boltzmann Institute for Rheumatology and Balneology,
Vienna, Austria,
*CIinical Department for Blood Group Serology, University
of Vienna, Vienna, Austria
67. DETECTION OF RED FLUORESCENT MICROPHERES USING AN ALL-
SOLID-STATE FLOW CYTOMETER
Stefan Niehren1, Wolfgang Kinzelbach1, Stefan Seeger2,
Jürgen Wolfrum2
1 Institute of Environmental Physics, University of
Heidelberg, Im Neuenheimer Feld 366, 69120 Heidelberg
2 Institute of Physical Chemistry, University of Heidelberg,
Im Neuenheimer Feld 253, 69120 Heidelberg
68. FLOW CYTOMETRIC ANALYSIS OF T CELL REPERTOIRE: A RAPID
METHOD FOR PRODUCTION AND CHARACTERIZATION OF MONOCLONAL
ANTIBODIES AGAINST THE HUMAN a/beta T CELL RECEPTOR
C. Otto, F. Struck, K. Littmann-JanGen, J. Collins, A.
Lingnau, K.E.J. Dittmar
Gesellschaft fur Biotechnologische Forschung (GBF), Dept.
of Applied Genetics, D-38124 Braunschweig, FRG
69. BINDING TO LEUKOCYTES AND INTACT BIOLOGICAL ACTIVITY OF A
FLUORESCENT LABELED LMM-HEPARIN
L. Piazolo, J. Harenberg, R. Malsch and D. L. Heene
1st. Department of Medicine, Medical University Clinic,
Theodor-Kutzer Ufer, 68167 Mannheim, Germany
70. RESOLUTION EFFECTS IN "PRACTICAL" LIGHT MICROSCOPY AND THE
APPLICATION OF A 2pi TILTING DEVICE
Bernd Rinke, Joachim Bradl, Bernhard Schneider, Michael
Hausmann, Christoph Cremer
Institute of Applied Physics, University of Heidelberg,
D-69120 Heidelberg, Germany
71. CELL CYCLE OR PROLIFERATION DEPENDANCY OF DIFFERENTIAL
EXPRESSION OF CD34 BY HUMAN ENDOTHELIAL CELLS?
O. Röhrig, C.L. Klein, T. van Kooten, F. Bittinger,
C.J.Kirkpatrick
Institute of Pathology, University of Mainz, Germany
72. FLOW CYTOMETRIC DNA ANALYSIS OF PROSTATE TUMORS
K. Romanakis1, B. Wullich2, K. Becker3, B. Kopper3, and H.
Zankl1
1University of Kaiserslautern, Dept. Human Biology and Human
Genetics, 67663 Kaiserslautern, 2University of the saar, Dept.
of Urology, 68141 Homburg/Saar, 3Universitv Hospital of
Kaiserslautern, 67655 Kaiserslautern
73. DNA-CYTOMETRY AND KARYOMETRIE OF CERVICAL INTRAEPITHELIAL
NEOPLASIAS
M. Ruhnke, A. Coumbos W. Kühn
Department Gynecol. Obstet., Klinikum Benjamin Franklin, Free
University of Berlin, Germany
74. EXPRESSION OF ADHESION MOLECULES OF THE IMMUNOGLOBULIN
SUPERFAMILY ON HUMAN RENAL CARCINOMA CELL LINES.
Schardt, C., Gerharz, CD., Gabbert, HE.
Institute of Pathology, Düsseldorf, Germany
75. MORPHOMETRIC AND DENSITOMETRIC IMAGE ANALYSIS ON BLADDER
CARCINOMA CELLS STAINED WITH FLUORESCENT DYES
Wolfgang Schildbach, Elmar Endl, Pia Steinbach, Gabriele
Fauser, Josef R.Aschoff and Ferdinand Hofstädter
Institute of Pathology, University of Regensburg,D-93042
Regensburg, Germany
76. ENDOMITOTIC CHROMOSOMES IN THE NURSE CELLS OF CHRYSOMYA
RUFIFACIES
Michael Schöttke
Zoologisches Institut der Universität Kiel, Olshausenstr. 40,
D-24098 Kiel
77. TELEMICROSCOPY - A TOOL FOR THE REMOTE EVALUATION OF
CYTOLOGICAL MATERIAL
Schwarzmann P., Schmid J., Schnörr C., Binder B.
Inst. für Physikalische Elektronik, University Stuttgart,FRG
78. FLOW CYTOMETRIC ANALYSIS OF CELL CYCLE PERTURBATIONS AND
MlCRONUCLEI AFTER UV B- AND gamma-IRRADIATION
Weller E.M.1, Hain J.1, Jung T.1, Kinder R.2, Köfferlein M.3,
Burkart W.1 and Nüsse M.2
1BfS-lnst. f. Strahlenhygiene, 2GSF-AG Durchflusszytometrie, 3
GSF Inst. f. Biochemische Pflanzenpathologie, 85762 Oberschleiss-
heim, Germany
79. FLOW SORTING OF BACTERIA FOR PCR-ANALYSIS
G. Wallner1, S. Spring2, B. Fuchs2, W. Beisker1, and R. Amann2
1 GSF-Forschungszentrum, AG Durchflußzytometrie, D-85764
Oberschleißheim
2 TU München. Lehrstuhl fur Mikrobiologie, D-80290 München
80. OXIDATIVE STRESS AND NO* INDUCE APOPTOSIS IN RAT MYOGENIC
CELLS
U.K.Zettl1,2, E.Mix1, J.Zielasek2, M.Stangel3, H.Meyer-
Rienecker1, K.V.Toyka2, H.-P.Hartung2, R.Gold2
1Dept. of Neurology, Hosp. Nerv. Dis., Universität Rostock,
2Dept. of Neurology, Universitat Würzburg, 3Dept. of
Neurology, Freie Universität Berlin, Germany
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