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The abstract are printed in the: Proceedings of the DGZ Heidelberg Meetings, DKFZ, Heidelberg 1996, ISSN 0949 - 5347
2. IMPROVED SINGLE LASER MEASUREMENT OF TWO CELLULAR
ANTIGENS AND DNA-PLOIDY BY THE COMBINED USE OF PROPIDIUM
IODIDE AND TO-PRO-3 IODIDE
Willem E. Corver, Gert Jan Fleuren and Cees J. Cornelisse
Department of Pathology, Faculty of Medicine, Leiden University, Leiden, the
Netherlands
3. GRADE OF ANEUPLOIDY (5c- AND 9c-EXCEEDING RATE) AS A
PROGNOSTIC MARKER FOR BREAST CANCER
Coumbos, A., Ruhnke, M., Yildirim, S. and Kühn, W.
Dept. of Obstetrics and Gynecology, Klinikum Benjamin Franklin, Free
University of Berlin,Hindenburgdamm 30, 12200 Berlin, Germany
.4 THE VALUE OF FLOW CYTOMETRY IN STUDYING
MALIGNANT GLIOMAS
V. Ehemann, B. Eifert*, K. Munkel*, A. Lange, H. F. Otto
Institute of Pathology, and Department of Neurosurgery*, University of
Heidelberg, Germany
5. STANDARDIZATION OF FLOW-CYTOMETRIC DUAL PARAMETER-DNA MEASUREMENTS
ON HUMAN COLON CARCINOMA
Ruth Knüchel, H.Zirngibl*, F.Hofstädter
Institute of Pathology and Dept. of Surgery, University of Regensburg,
Germany
6. METRIC ANALYSIS OF MITOCHONDRIAL FUNCTION OF
ONCOGENE-TRANSFORMED FIBROBLASTS ISOLATED FROM 2D- AND
3D-CULTURE
L.A. Kunz-Schughart1,2, R.C. Habbersett1, and J.P. Freyer1
1Los Alamos Natl. Lab., Life Sciences Division, Los Alamos, NM 87545, USA
and 2Institute of Pathology, University of Regensburg, 93042 Regensburg,
Germany
7. CHARACTERISATION OF APOPTOSIS IN LUTEAL CEllS FROM EARLY
OVARIAN CYCLE STAGES BY FLOW CYTOMETRY
Bertold Löhrke, Ralf Pöhland, Torsten Viergutz
Research Institute of Animal Biology, Dummerstorf-Rostock
8. A NOVEL, MEMBRANE RECEPTOR BASED RETROVIRAL VECTOR FOR
FANCONI ANEMIA GROUP C GENE THERAPY
Andreas W. Machl, Simone Planitzer, Manfred Kubbies
Boehringer Mannheim GmbH, Biotechnology Therapeutics Research Center,
82377 Penzberg, Germany
9. ANEUPLOIDY OF HUMAN GRANULOSA CELLS IN FOLLICULAR FLUIDS
OF IN-VITRO FERTILIZATION (IVF) PATIENTS
P. Melsheimer1, K. Grunwald2, K. Feldmann2, K. Klinga2, T. Rabe2, B.
Runnebaum2, H.H. Rummel1
1 Abteilung fur Gynäkologische Morphologie, Universitäts-Frauenklinik,
Vossstr. 9, D69115 Heidelberg,
2 Abteilung fur Gynäkologische Endokrinologie, Universitäts-Frauenklinik
Heidelberg
10. THE INCIDENCE OF ANEUPLOIDIES IN MULTIPLE MYELOMA DOES NOT
CORRELATE WITH STAGE OF DISEASE
R. Nowak, U. Oelschlägel, U. Range and G. Ehninger
Medical Clinic I, Technical University Dresden, 01307 Dresden
11. NON-SYNGENEIC CELL MODELS FOR SCREENING OF DNA-REPAIR
RELEVANT GENES IN FANCONI'S ANEMIA VIA DIFFERENTIAL DISPLAY
TECHNIQUE
Simone Planitzer, Andreas W. Machl and Manfred Kubbies
Boehringer Mannheim GmbH, Biotechnology Therapeutics Research Center,
82377 Penzberg, Germany
12. ALTERATIONS IN CELLULAR MITOCHONDRIAL POTENTIAL UNDER
INFLUENCE OF APOPTOSIS INCUCING DRUGS
Pogrebniak A, Stoetzer OJ, Wilmanns W, Nüssler V
GSF-Research Center for Health and Enviroment, Institute for Clinical
Hematology, Munich
13. DNA DIPLOIDY - A RARE FEATURE OF OVARIAN CARCINOMA?
Reich O, Pürstner P.
Department of Obstetrics and Gynecology, University of
Graz, Austria
14. DISCRIMINIATION OF OVARIAN TUMORS BY DNA CYTOMETRY
M.Ruhnke, A. Ditzenbach and W. Kühn
Department of Gynecologic Morphology, Academic Medical Center Benjamin
Franklin. Free University of Berlin, Hindenburgdamm 30, D-12200 Berlin
15. ANGIOGENESE IN OVARIALKARZINOMEN: ERSTE ERFAHRUNGEN MIT
BILDANALYTISCHER QUANTIFIZIERUNG DER ENDOTHELIALEN
IMMUNOREAKTIVITAET UND IHRER MOEGLICHEN PROGNOSTISCHEN
BEDEUTUNG
W. Schöll, O. Reich, D. Pieber, F. Gücer
Geburtshilflich-Gynäkologische Univ.-Klinik, Graz
16. NUMERICAL ABERRATIONS OF CHROMOSOME 17 IN DIPLOID AND
ANEUPLOID TUMORS.
Schnekenbühl S. Polackova J, Nagel E, Hemmer J.
Divison of Tumor Biology, University of Ulm
17. PROLIFERATION KINETIC IN GASTRIC CANCER"IN VIVO"
A. Sendler* , K.-P. Gilbertz , A. Rhein , K. Matzen*, U. Fink*, l. Becker+, D.
van Beuningen
*Chirurgische Klinik und Institut für Pathologie der TU München, Klinikum
rechts der Isar, 81644 München, Institut für Radiobiologie, Akademie des
Sanitäts- und Gesundheitswesen der Bundeswehr, 80901 München, Germany
18. FLOW CYTOMETRIC ANALYSIS OF LYMPHOMA CELL
IMMUNOPHENOTYPE USING KNOWLEDGE BASED CLUSTER SOFTWARE
S. Barlage, G. Rothe, G. Schmitz
Institute for Clinical Chemistry and Laboratory Medicine, University of
Regensburg, Germany
19. FACS-ANALYSIS OF SELECTIVE SKIN INFILTRATING BLOOD CELLS
DUE TO AN ANTIGENIC CHALLENGE IN VIVO
B&oouml;hm I., Bauer R.
Universitäts-Hautklinik Bonn, Sigmund-Freud-Str. 25, D-53105 Bonn
20. CD69 EXPRESSION AS A MEASURE FOR CHANGES IN PROLIFERATION
OF LYMPHOCYTES IN HIV-AB+ PATIENTS AND PATIENTS WITH CFIDS
Janssen M., Braun P. and Knechten H.
PZB Aachen, Blondelstr. 9, 52062 Aachen, Germany
21. BLOCKING OF THE HIV-1 ENVELOPE GLYCOPROTEIN (GP120)
BINDING TO CD4+ T LYMPHOCYTES BY ANTI-HIV MONOCLONAL
ANTIBODIES
Kröpelin, M., Süsal, C., Daniel, V., and Opelz, G.
University of Heidelberg, Institute of Immunology, 69120 Heidelberg, Germany
22. CYTOMETRIC ANALYSIS OF ANTIBODY-SECRETING CELLS
R. A. Manz1, A.Thiel1, S. Miltenyi2 and A. Radbruch1,
1Institute for Genetics, University of Cologne, Germany, 2Miltenyi Biotec GmbH, Bergisch Gladbach,
Germany
23. An IMPROVED FLOW CYTOMETRIC WHOLE BLOOD ASSAY FOR
RETICULATED PLATELETS
G.B. Matic, G. Rothe, G. Schmitz
Institute of Clinical Chemistry and Laboratory Medicine, University of
Regensburg, Germany
24. IN VITRO INVESTIGATIONS OF LYMPHOCYTE HOMING IN
INFLAMMATORY RHEUMATIC DISEASES USING FLOW CYTOMETRY
AND IMAGE ANALYSIS
J. Neumüller (1), J. Menzel (2), A. Dunky (3)
(1) Ludwig Boltzmann Institute for Rheumatology and Balneology, Vienna, (2)
Institute of Immunology, University of Vienna, (3) 5th Department for Internal
Medicine, Wilhelminenspital, Vienna, Austria
25. DETECTION OF CYTOKERATIN 8-18 AND P53 POSITIVE CELLS BY
FLOW CYTOMETRY IN HUMAN RENAL CARCINOMA
Eugen Plas, Ruth Jilch*, Sonja Thury*, Michael Fischer*, Heinz Pflüger
Dept. of Urology and LBI for Urology and Andrology, Lainz Hospital
*Dept. of Laboratory Medicine, Lainz Hospital Vienna, Austria
26. Institute for Genetics, University of Cologne, Germany, #Miltenyi Biotec
GmbH, Bergisch Gladbach, Germany.
IFN-y AND IL-10 CAN BE DETECTED ON THE SURFACE OF SECRETING
CELLS BY MAGNETOFLUORESCENT LIPOSOMES: SPECIFIC MARKERS
FOR CYTOKINE SECRETION
A. Scheffold&, M. Assenmacher&. J. Schmitz#, S. Miltenyi# and A. Radbruch&
27.IMMUNOLOGICAL ALTERATIONS DURING PEDIATRIC CARDIAC AND
VASCULAR SURGERY: THE INFLUENCE OF THE CARDIO-PULMONARY
BYPASS (CPB)
Tarnok A., Hambsch J., Kinzel P., Borte P., Sack U., Schneider P.
Pediatric Cardiology, Heart Center Leipzig - University Hospital - Russenstr.
19, 04275 Leipzig, Germany
28. MEASUREMENT OF PROTEIN-PROTEIN-INTERACTION BY FLOW
CYTOMETRY USING FLUORESCENT MICROBEADS
Tarnok A.1, Nöhrenberg U.2, Schuhmacher S.2, Vollmer H.J.2, Rathjen F.2
Pediatric Cardiology, Heart-Center Leipzig - University Hospital - Russenstr. 19,
Leipzig
2 Max-Delbruck-Center for Molecular Medicine, Berlin-Buch, Germany
29. FLOW CYTOMETRIC CHARACTERIZATION OF HUMAN TROPHOBLASTS
AFTER ISOLATION FROM FRESH PLACENTAL TISSUE
Thieme B., Koop F., Harbeck N., Kolben M., *Ugele B., Schneider KTM.,
Graeff H. and Schmitt M.
Departments of Obstetrics & Gynecology, Technical University, * Ludwig-
Maximilians-University, Munich, Germany
30. CHARACTERIZATION OF A PATIENT WITH REDUCED PLATELET CD36
EXPRESSION AND ENHANCED GLYCOPROTEIN Ib-lX EXPRESSION.
A. Zwick, E. Orso, J. Stöhr, E. Ajzner, S. Barlage, W. Drobnik, G. Rothe, C.
Aslanidis and G. Schmitz.
Institute for Clinical Chemistry and Laboratory Medicine, University of
Regensburg,D-93042 Regensburg, Germany
31. Microscope control, image acquisition and visualization in a network
environment: towards "online", long distance telemicroscopy
by Matthias Nagorni, Joachim Bradl, Bernhard Schneider,
Michael Hausmann, Christoph Cremer
Institute of Applied Physics, University of Heidelberg,
Albert-Ueberle-Str. 3-5, D-69120 Heidelberg
32. Axialtomographical Microscopy for High Resolution Studies of the
Interphase Genome Organization
B.Rinke1, P.Edelmann1, J.Bradl1, A.Esa1, B.Schneider1, M.Hausmann1,
C.Cremer1,2
Institute of Applied Physics, University of Heidelberg 2
Interdisciplinary Centre for Scientific Computing (IWR), University of
Heidelberg
33. A NEW PRINCIPLE IN CONFOCAL MICROSCOPY:
INVARIABLE LIGHTPATH LASER-SCANNING MICROSCOPY
VON STELDERN DU,1, Milicev M., 2
1Biotechnologie CONSULTING, D-69 1 8 1 LEIMEN 2 MG MICROSCOPE
GMBH, D-69245 BAMMENTAL
34. CONFOCAL 3D-MICROSCOPY: ACQUISITION, DOCUMENTATION AND
PRESENTATION
P.J.,S. Hutzler,
GSF-Forschungszentrum, Oberschleißheim
35. MORPHOMETRIC ANALYSIS OF AgNOR PROTEINS FOR CELL DOUBLING
TIME EVALUATION IN TUMOR PATHOLOGY
Massimo Derenzini
Dipartimento di Patologia Sperimentale, Universita di Bologna Italy
36. QUALITY ASSESSMENT FOR IMMUNOPHENOTYPING OF LEUKEMIAS
AND LYMPHOMAS
J.W. Gratama1 and J.C. Kluin-Nelemans 2, for the Dutch Cooperative Study
Group on Immunophenotyping of Hematological Malignancies (SIHON)
1 Department of Clinical and Tumor Immunology, Daniel den Hoed Cancer
Center, Rotterdam, and Department of Hematology, University Hospital, Leiden, the Netherlands
37. EXPERIENCES FROM COLLABORATIVE STUDIES ON QUALITY
ASSURANCE OF FLOW CYTOMETRIC DNA MEASUREMENTS
Friedrich J. Otto, Ulrike Westhoff
Fachklinik Hornheide, Abteilung fur Tumorforschung, D-48157 Münster
38. EUROPEAN CONSENSUS PROTOCOL FOR THE FLOW CYTOMETRIC
CHARACTERIZATION OF PLATELET FUNCTION
G. Rothe for the European Working Group on Clinical Cell Analysis - Task
Force on Platelet Analysis (J. Kappelmayer, R. Lenkei, G. Rothe, G. Schmitz)
39. USE OF ELECTRONIC CELL COUNTER ANALYSER SYSTEMS FOR
QUALITY CONTROL
5W-Rule: Who did what, when, with what and why? (GLP)
Schärfe System GmbH, Emil-Adolff-Straße 14, D-72760 Reutlingen
40. EXTERNAL QUALITY ASSESSMENT SCHEME "RETICULOCYTES"
(INSTAND E.V.)- A THREE YEARS EXPERIENCE
Stefan Serke,
Virchow-Klinikum der Humboldt-Universität zu Berlin, Abteilung
Innere Medizin und Poliklinik, Hämatologie und Onkologie, Augustenburger
Platz 1, 13353 Berlin, FAX: +49.30.4505.3914
41. FLOW CYTOMETRY WITH PHENOL DEGRADING BACTERIA AFTER IN
SITU HYBRIDIZATION AND DAPI STAINING
C. HERRMANN
Abteilung Biotechnologie, Fakultät für Biowissenschaften, Pharmazie
und Psychologie, Universitaml;t Leipzig, Permoserstr. 15, 04318 Leipzig
42. LIGHT SCATTER MEASUREMENTS OF BACTERIA: MODEL
CALCULATIONS AND EXPERIMENTS
Andreas Lösche
Projektgruppe Biosignale, Abteilung Biotechnologie, Fakultät für
Biowissenschaften, Pharmazie und Psychologie, Universität Leipzig,
Permoserstr. 15, D-043 18 Leipzig
43. MICROORGANISMS IN CONCENTRATED FRUIT JUICES
Moustafa M., Wartenberg R. and Sachsse W.
Depart. of cytogenetic Univ. of Mainz
44. ASSESSMENT OF BACTERIAL VIABILITY STATUS BY FLOW
CYTOMETRY AND SINGLE CELL SORTING
G. Nebe-von Caron*, P. Stephens and R.A.Badley
Unilever Research, Colworth Laboratory, Sharnbrook, Beds., MK44 1LQ, UK
45. GERMINATION AND OUTGROWTH OF SPORES CHARACTERIZED BY
FLUORESCENT PROBES AND FLOW CYTOMETRY
G. Nebe-von Caron* and W.A. Anderson,
Unilever Research, Colworth Laboratory. Sharnbrook. Beds., MK44 1LQ United Kingdom
46. FLOW CYTOMETRY OF MESOPHYLL AND BUNDLE SHEATH
CHLOROPLASTS OF VARIOUS PLANTS WITH C4 PHOTOSYNTHESIS
E. Pfündel 1,2 and A. Meister1,
1 Institut für Pflanzengenetik und Kulturpflanzenforschung, Corrensstr. 3, D-
06466 Gatersleben.
2 Universität Leipzig, Institut fur Botanik, Johannisallee 21, D-04103 Leipzig
47. THREE-LASER FLOW CYTOMETRY FOR SIMULTANEOUS
MEASUREMENT OF PHOTOSYNTHESIS PIGMENTS AND PROTEIN
CONTENT OF PHYTOPLANKTON POPULATIONS IN LAKES AND RIVERS
Schäfer,H.1,2; Bruckmeyer,B.1,2;Steinberg, C.2 and Beisker, W.1
(1): GSF-Durchfluszlig;zytometrie Ingolstädter Landstr 1, D-85764 Neuherberg, Germany
(2): Institut fur Gewässerökologie und Binnenfischerei Müggelseedamm 310,
D-12587Berlin, Germany
48. NON-INVASIVE BIOPROCESS MONITORING: POPULATION DYNAMICS
Bernhard Sonnleitner
Institut für Biotechnologie, ETH Zürich
49. OUTGROWTH AND VITALITY DISTRIBUTIONS OF INJURED
LACTOBACILLUS PLANTARUM CELLS ASSESSED BY FLOW CYTOMETRY
Ueckert J.and Ter Steeg,P.F.
Unilever Research Laboratorium P.O. Box 114, 3130 AT Vlaardingen, NL
50. FLUORESCENCE LIFE-TIME MEASUREMENTS IN FLOW CYTOMETRY
PRINCIPLES AND FIRST APPLICATIONS
Beisker, K. and Klocke, A.
GSF-Durchflußzytometrie Ingolstaedter Landstr 1, D-85764 Neuherberg, Germany
51. COMPREHENSIVE MONITORING OF CELLS IN CULTURE: THE
PHYSIOCONTROL-MICROSYSTEM
M. Brischwein a*, A. Schwinde a, W. Baumann a, R. Ehret a, P. Seidl b and B.
Wolf a
a AG Medizinische Physik und Elektronenmikroskopie, Institut für
Immunbiologie, Universität Freiburg, Stefan-Meier-Str. 8, 79104 Freiburg;
Germany,
b Physikalisch Technische Studien GmbH, Leinenweberstr. 16,, 79108
Freiburg, Germany
Keywords: cellular biosensor, Physiocontrol-Microsystem, transducer array
52. MEASUREMENT OF INTRACELLUAR ENZYME ACTIVITY BY THE AID OF
MULTIPARAMETRIC FLOW CYTOMETRY
Rolf Eckhardt,
Coulter Electronics GmbH, Europark Fichtenhain B13, 47807 Krefeld
53. FORMATION OF CELLULAR AGGREGATES DURING ROUTINE
IMMUNOSTAINING: RELEVANCE OF THE FCY RECEPTOR IIA
POLYMORPHISM FOR CLINICAL FLOW CYTOMETRY
J.W. Gratama,1 R. v.d. Linden,l C. de Beukelaer,1 J.G.J. van de Winkel 2 and
R.L.H. Bolhuis1
Department of Clinical and Tumor Immunology, Daniel den Hoed Cancer
Center, Rotterdam
and 2 Department of Immunology, University Hospital, Utrecht, the
Netherlands
54. LABELING AND ANALYSIS OF RECOMBINANT HIRUDUIN USING FLOW
CYTOMETRY
Malsch, L. Piazolo, W. Lianchun, A. Timmermann and J. Harenberg
I. Medizinische Klinik, Fakultät für klinische Medizin Mannheim der Universität
Heidelberg, Theodor Kutzer Ufer, 68167 Mannheim
55. ANALYSIS OF THE ROLE OF THE FAC-GENE IN THE REGULATION OF
THE CELL CYCLE AND APOPTOSIS WITH A NOVEL FLOW CYTOMETRIC METHOD
Martin Poot, Frank Kruyt2, Hans Joenje2, Victoria L. Singer and Richard P.
Haugland.
Molecular Probes, Inc., Eugene, OR 97402, U.S.A. und Department
of Human Genetics 2, Free University of Amsterdam,1081 BT
Amsterdam, The Netherlands
56. DISSECTION OF APOPTOTIC PATHWAYS IN LIVE CELLS BY
MITOTRACKER RED CM-XROS AND SYTO DYE FLOW CYTOMETRY
Martin Poot, Lisa L. Gibson, Victoria L. Singer and Richard P. Haugland.
Molecular Probes, Inc., Department of Biosciences, Eugene, OR 97402, U.S.A.
57. EXCIMER FLUORESCENCE COMPARED TO DEPOLARIZATION IN THE
FLOW CYTOMETRIC CHARACTERIZATION OF LATERAL MEMBRANE MOBILITY
G. Rothe, B. Schäfer, G. Schmitz
Institute for Clinical Chemistrv. University of Regensburg, Germany
58. FLOW CYTOMETRY OF ALKALINE AND ACID PHOSPHATASE
ACTIVITIES - COMPARISON OF THE DIAZOIC COUPLING WITH THE
METHYLUMBELLIFERON METHOD
E. Severin und E. Seidler,
Institut fur Strahlenbiologie der Universität Münster,
Robert-Kochstr. 43, D-48149 Münster
59. PHASE-SENSITIVE FLOW CYTOMETRY: LIFETIME-BASED TECHNOLOGY
FOR ANALYZING CELLS\CHROMOSOMES LABELED WITH FLUORESCENT PROBES
John A. Steinkamp
Life Sciences Division, Los Alamos National Laboratory, Los Alamos, NM
87545, USA
60. PROGNOSTIC SURVIVAL ESTIMATION OF COLORECTAL CANCER
PATIENTS BY CLASSIF1 ANALYSIS OF MULTIPARAMETER DATA FROM
G6PDH OXYGEN INSENSITIVITY TEST, SOD AND LIPID PEROXIDATION
DETERMINATION ON HISTOPHATOLOGICAL CRYOSECTIONS.
G.Valet1), B.E.M. Van Driel2), H.Lyon3), U.Hansen3), J.Song2), C.J.F. Van
Noorden2)
1) Max-Planck-lnst.Biochemie, 82152 Martinsried, Germany, 2) Lab.Cell
Biol.and Histol, Academic Med.Center, Univ.Amsterdam, NL, 3) Dep.Pathol.,
Kobenhavns Kommunes Hvidovre Hosp., Univ.Copenhagen, Denmark
61. FLOW CYTOMETRIC ASSESSMENT OF SHOCK WAVE INDUCED
ALTERATIONS OF PLASMA MEMBRANE POTENTIAL.
G. Woelfl, J. Bette, E. Endl, F. Hofstädter,
Institute of Pathology, Univeristy of Regensburg, Germany
62. HIGH SPEED FISH FOR REPETITIVE AND PAINTING PROBES
M. DURM1,3, D. WOLF1, K. ALDINGER1, F.-M.HAAR1,M. HAUSMANN1, C.
CREMER1,2
63. FAST FISH DETECTION AND AUTOMATED DIGITAL IMAGE ANALYSIS OF
NUMERMICAL CHROMOSOMAL ABERRATIONS IN BONE MARROW AND
PERIPHERAL BLOOD INTERPHASE NUCLEI OF HEMATOONCOLOGICAL
PATIENTS
A. Esa1. L. Trakhtenbrot2. M. Hausmann1, J. Ben-Bassat2, C. Cremer1
1 = Institute of Applied Physics, University of Heidelberg, Albert-Ueberle-Str. 3-
5, D-69120 Heidelberg, F.R. Germany
2 = The Institute of Hematology, The Chaim Sheba Medical Center, Tel
Hashomer, Israel
64. AUTOMATIC OBSERVATION OF THE DROPLET BREAKOFF POINT
Joachim W. Ellwart. Ingolf Karls*
GSF-National Research Center for Environment and Health, Institute of
Experimental Hematology, Marchioninistr. 25, D-81377 Munich, Germany
*Siemens AG, Munich, Germany
65. CELL CULTURE TESTING OF BONE REPLACEMENT MATERIALS GLASS
IONOMER CEMENT AND POLYMETHACRYLATE
Dr.Dr. S. Hassfeld, Dr. R. Frombach, Dr.Dr. R. Wiedenmann, PD Dr.Dr. J.
Zöller
Oral and Maxillofacial Surgery, Ruprecht-Karls-University Heidelberg Im
Neuenheimer Feld 400, 69120 Heidelberg, Germany
66. CHANGES OF LYMPHOCYTE SUBSETS AND THEIR CELL HOMING
RECEPTOR CAPACITY IN HUMAN BLOOD AFTER TOTAL SLEEP DEPRIVATION
T.O. Kleine*, W. Schreiber**, J. Albrecht***
*Funktionsbereich Neurochemie,**Klinik fur Psychiatrie, Med. Zentrum fur Nervenheilkunde, Philipps-Universitat
Marburg; ***Becton Dickinson, Heidelberg
67. TRANSFER OF LYMPHOCYTES THROUGH THE BLOOD/CSF BARRIER OF
HUMANS
T.O. Kleine*, H.J. Werner*, J. Albrecht**
*Med. Zentrum fur Nervenheilkunde, Funktionsbereiech Neurochemie,
Philipps-Universitat Marburg
** Becton Dickinson, Heidelberg
68. DETECTION OF H202/L-HISTIDINE INDUCED CHROMOSOME
ABERRATIONS BY MEANS OF SLIT-SCAN FLOW FLUOROMETRY
Heiko Lentfer, Dietmar Wolf, Martin Crone, Klaus Aldinger, Michael
Hausmann, Christoph Cremer
Institute of Applied Physics, University of Heidelberg, Albert-Ueberle-Str. 3-5,
D-69120 Heidelberg, F.R. Germany
69. A NEW FLOW CYTOMETRIC ASSAY FOR BASOPHIL DEGRANULATION
Thomas Nebe, Karin Hartmann and Waltraut Pfirrmann
Institut für Klinische Chemie. Klinikum Mannheim, D-68135 Mannheim
70. COMPARISON OF DIFFERENT METHODS, TARGETS AND INDUCERS OF APOPTOSIS
Thomas Nebe1, Jutta Wucher1, Monika Bühl2, Gerolf Maier1, Ingrid Brechtel1
and Werner Hirt2
1 Institut fur Klinische Chemie, Klinikum Mannheim, D-68135
Mannheim and 2 ORPEGEN Pharma, D-69115 Heidelberg, Germany
71. COMPARISON OF DIFFERENT TECHNIQUES TO ANALYSE MULTI-DRUG
RESISTANCE
Thomas Nebe1, Susan Kranzpiller1, Verena Zunftmeister1 M. Cianfriglia3, and
Alexandra Dorn-Beineke2
1 Institut für Klinische Chemie, Klinikum Mannheim, D-68135 Mannheim,
2 Labor Prof. Seelig, Kriegstral3e 99, D-76133 Karlsruhe, Germany and 3 Inst.
f. Sanita Superiore di Roma, Italy
72. PROLIFERATION VERSUS APOPTOSIS: THE BIOLOGICAL ACTIVITY OF
THYMIC PEPTIDES ON LYMPHOCYTES OF HEALTHY DONORS AND HIV PATIENTS
Thomas Nebe1, Gerolf Maier, Karin Hartmann, Ingrid Brechtel and Gerhard
Becker2
1 Institut fur Klinische Chemie, Klinikum Mannheim, D-68135 Mannheim and
2 Sanorell Pharma,D-72270 Baiersbronn, Germany
73. PROLIFERATION, APOPTOSIS OR PHAGOCYTOSIS: THE SUPPRESSIVE
EFFECT OF AUTOANTIBODIES ON LYMPHOCYTES OF HEALTHY DONORS AND HIV PATIENTS
T.Nebe1, G.Maier1, M.Müller1, I.Brechtel1, K.Hartmann1, F.Thienel2,
B.Buchholz3, K.Friese4, A.Willer5 and K.P.Becker6
1Inst für Klinische Chemie, 2I.Med.Klinik, 3Kinderklinik, 4Frauenklinik
5III.Med. Klinik, 6Institut fur Mikrobiologie und Hygiene, Klinikum Mannheim,
D-68135 Mannheim, Germany
75. INDUSTRIAL PROCESS ANALYSIS: FLOW CYTOMETRY IN THE BREWING
TECHNOLOGY
Petzold, Louise1; Müller, Susann1, Hutter, Karl-Josef2, Bley, Thomas 3
1 Universität Leipzig, Abteilung Biotechnologie, Permoserstr.15, Leipzig
2 DKFZ Heidelberg, Im Neuenheimer Feld 280, Heidelberg
3 TU Dresden. Institut Lebensmittel- und Bioverfahrenstechnik, Dresden
76. ANALYSIS OF CHROMOSOMAL IMBALANCES IN BIOPSIES FROM ORAL
SQUAMOUS CELL CARCINOMAS AND PRECURSOR LESIONS BY A
COMBINATION OF MICRODISSECTION, UNIVERSAL DNA-
AMPLIFICATION AND COMPARATIVE GENOMIC HYBRIDIZATION (CGH)
Martin Scheer1,2,4; Ruthild Weber 2,4; Christof Hofele 1; Stefan Joos 4; 1.
Antonio Born 3; Peter Lichter 4; Joachim Zöller 1; Thomas Cremer 2,5
1 Department of Oral and Maxillofacial Surgery, 2 Institute of Human Genetics
and 3 Institute of Pathology, University of Heidelberg, 4 German Cancer
Research Center, 69120 Heidelberg, Germany and 5 Institute of Anthropology
and Human Genetics, 80333 München. Germany
77. DISTANCE MEASUREMENTS OF SINGLE TARGETS IN INTERPHASE
NUCLEI BY WAVEFIELD MICROSCOPY
Bernhard Schneider 1, Joachim Bradl 1, Ingolf Kirsten 1, Matthias Nagorni 1, Bernd
Rinke 1, Michael Hausmann 1, Christoph Cremer1,2
1, Institut für Angewandte Physik, Universität Heidelberg, Albert-Ueberle-Str. 3-
5, D-69120 Heidelberg
2 Interdisziplinäres Zentrum far wissenschaftliches Rechnen (IWR), Universität
Heidelberg.
key words:
distance measurements in fluorescence microscopy, axialtomography
78. HISTKOM - FIELDTEST IN GERMANY FOR TELEPATHOLOGY AND
TELECYTOLOGY
P. Schwarzmann, B. Binder, J. Burkart, J. Schmid
Institut fur Physikalische Elektronik, Universität Stuttgart
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