D cyclins

Michael Fox (MFox@vines.ColoState.EDU)
Mon, 15 Jul 96 9:26:31 MDT

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Does anyone out there have experience measuring D cyclins in CHO cells? I am
trying to measure cyclin D1 in CHO cells using a Pharmingen human antibody
that should cross react with mouse and hamster, but we get no staining of the
cells. Do CHO cells express cyclin D1 (or other D cyclins)? Does anyone
know of another source of antibodies against D cyclins that have been tested
against hamster cells?

Thanks for any input.

Mike Fox
Colorado State University

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