Re: pulse width
kukuruga%kasle1.dnet.wayne.edu@rocdec.roc.wayne.edu
Thu, 1 Aug 1996 12:24:50 -0400
My two cents . . .
Forward angle scatter is, relatively speaking, a HUGE signal compared
to others we encounter in flow. Therefore, while we may perceive a
"significant" difference amongst cells microscopically, their FALS may
be unremarkable (e.g, stimulated lymphocytes which show large 90 degree
scatter changes while changing only slightly in FALS).
Also . . . intuitively, as a cell "swells" it may also change with respect
to refractive characteristics, and the net change is less than perhaps
expected.
MAK.
CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories
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If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL,
Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu
EMAIL robinson@flowcyt.cyto.purdue.edu
