three color analysis
Antony Bakke (bakkea@ohsu.EDU)
Thu, 07 Dec 95 10:57:28 PST
Instead of PI in FL2 and Cy3 in FL3, you can use 7AAD in FL3 and PE
in FL2. (see Schmid et al. Cytometry 12:279-285, 1991) However,
7AAD does not give low CVs - usually about 4-6%.
You can also use FITC, PE and PI according to a paper in Cytometry,
but this may require high levels of expression of the antigens
detected with FITC and PE: Corver, et al Cytometry 15: 117-128,
1994.
Tony Bakke (bakkea@ohsu.edu)
CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories
and distributed free of charge as an educational service to the cytometry community.
If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL,
Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu
EMAIL robinson@flowcyt.cyto.purdue.edu
