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Contact the good people at Phoenix Flow Systems. They sell this as a kit
which includes positive and negative controls. They can be reached at
800-886-3569 or email: info@phnxflow.com.
Lisa Adams
Cancer Research
Pharmacia & Upjohn
Kalamazoo, MI
______________________________ Reply Separator _________________________________
Subject: apoptosis
Author: "Hughes; EJ; Jane; Ms" <JHUGHES@ICH.UCT.AC.ZA> at INTERNET
Date: 4/22/97 11:30 AM
Dear Flowers
We are setting up an apoptosis assay using FITC anti BrdU ( the
method of Xun Li, Traganos and Darzynkiewicz) and I have a few
questions (I do hope not TOO basic!!)
1. Should one use an Isotypic control.
2.I want to immunophenotype first, should I lyse the RBC after
immunophenotyping and before DNA cleavage. (I wasn't sure from the
method whether Triton would lyse the cells as RBC lysis is not given
as a step)
3. What are the options for positive controls.
Thanks
Jane Hughes
Immunology Section
ICH Lab, Dept Paediatrics
Red Cross Children's Hospital
Rondebosch, Cape Town, South Africa
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web