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We have used fluo-3 and fura red on about 25 studies using
platelets. Platelets are actually harder to work with than some
other PBLs, so I imagine our experience may be helpful to you,
though not specific to neutrophils. I would also be interested in
comparing data. (We have not used calcium green, but once
tried the Oregon green 488, I think it's called)
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web