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I have had the same problem. If you want classy
graphics you have several options and none of
them are simple.
There are several freeware/shareware programs
that can read files created by Cell Quest.
FCS Assistant ($25 shareware for the Macintosh) can
read these files and convert them to ASCII.
You can then plot the data in the graphing program of
choice. I am not sure whether FCS assistant allows
you to save gated files. I havent used it much.
If you have a Windows machine, get WinMDI which
lets you save histograms in ASCII. It also can save
gated histograms and dot plots.
Both FCS Assistant and WinMDI can be accessed from
the U Mass flow cytometry software page.
http://www.bio.umass.edu/mcbfacs/flowcat.html
Another approach is to scan the plots
of interest and then use DataThief (Available from the
NIH Image site, I dont know the URL) to "steal" the
x-y coordinates. This is really useful if you dont
have acess to the original files and are stuck with
a lousy print out. Data Thief creates a text file that
can be read in any graphing program. This does not
work for dot plots.
Hope that helps
Regards
Murali Ramanathan
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
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