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Jim Weaver wrote:
> Under most circumstances the assumption of linearity will be
>reasonable, however we need to be aware of the occasional
>circumstances where the assumption will not be correct.
Since immunofluorescence intensity is generally proportional to Ag density
if one carefully controls the staining conditions - what is the
proportionality?
Unless otherwise stated, proportional implies a direct or linear relationship.
This would hold for MAbs directly labeled with a single fluorochrome (FITC),
but what about phycobiliproteins? Or streptavidin conjugates? For a MAb
conjugated to PE wouldn't the relationship of fluorescence to Ag density be
geometric?
td
-- ============================================================================== Thomas Delohery | Internet: t-delohery@ski.mskcc.org Manager, Flow Cytometry Core Facility | Phone: (212) 639-8729 Memorial Sloan-Kettering Cancer Center | Fax: (212) 794-4019 1275 York Ave. Box 98 | New York, NY 10021 | ==============================================================================
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
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