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1. Collect the peripheral blood samples in ACD (1:9 ratio) to eliminate any
aggreagation. If you are not using ACD in the pheresis procedure, I would
suggest adding to the pheresis collects also. During venipuncture, use as
large bore needle as possible and withdraw the sample with a 'slow and gentle
touch' so as not to activate the platelets during collection...I would not
suggest using any vacutainer tubes for sample collection unless you add the
sample after releasing the vacuum.
2. During the acquisition, if you want to look at stricly the platelets, set
both the FSC and SSC on log scale and the platelets will be displayed along
the diagonal in the center of the screen, with the RBC in the upper right
corner.
3. I would suggest including a monocyte/granulocyte marker with the platelet
activation markers to distinguish platelets from platelets that are attached to
mono/grans. A double check would be to prepare a slide of the sample left from
acquistion to morphological evaluate for any aggregates or platelets attached
to cells.
April G. Durett
Technology Transfer - Flow Cytometry
Blood & Marrow Transplantation
Univ TX MD Anderson Cancer Center
phone (713)792-4367
e-mail : adurett@notes.mdacc.tmc.edu@internet
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web