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I used to see similar increases when staining with FDA, as it leaked out
of the cells the medium surrounding the cells became fluorescent,
therefore increasing the signals.
We timed the analyses precisely so that each sample was analyzed exactly
5 minutes after staining.
Deb Berglund
Montana State University
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web