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>Greetings, everyone:
>
>I am in need of a method to block Fc receptors on human cells. We have
>antibodies (whole molecule) against a ligand on the surface of certain cells
>that we want to use to block binding of those cells to their target - a
>receptor on a human macrophage cell line. However, we need to prevent
>binding to the Fc receptors on the macrophages via the Fc portion of our
>antibodies. We prefer not to have to make F(ab')2 fragments of all of the
>antibodies we will be testing.
>
>I know that anti-CD16/32 monoclonals are available to block Fc receptors in
>the mouse, but as far as I have been able to find out, the anti-human CD16
>and CD32 monoclonals are not efficient as blocking agents.
>
>If you have an efficient method of blocking human Fc receptors, please let
>me know. And, thanks in advance.
\|/
(o o)
________________________________oOo__(_)__oOo_________________________________
___/\_ | Rafael Nunez mailto:rafaeln@vetvir.unizh.ch
/ o \/| | University Inst.for Virology http://www.unizh.ch/vetvir
/ _| | Winterthurerstr. 266a Telephone: (+41) 1 6358709
/_/\__/-\/ | 8057 Zurich SWITZERLAND Faximile : (+41) 1 6358911
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
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