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Let me throw my two cents in.
I don't sterilize the instrument!!!!!!!!!!!!
(That is with any harsh chemicals ecetera.)
I have a BDIS FacStar Plus. It comes with a filter housing on the sheath
tank.
The manufacturer recommends first using a pre filter and then a 0.45
micron filter.
I have installed a 0.22 micron filter piggy backed on the 0.45 micron
filter.
The sheath first passes through the pre filter then through the 0.45
micron and lastly through the 0.22 micron filter.
Thus the sheath filter is now filter sterilized to 0.22 microns.
To test for sterility I turn on the Sheath only and after about five
minutes I hold a Blood agar plate to collect the
sheath stream (@ 5 mls) and place the blood plate in aerobic and/or
anaerobic conditions and haven't seen any growth yet.
When I sort I do sterilize the sample input line with bleach followed by
sterile 0.22 micron filtered sterile dH2O.
Pb
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web