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I would question the efficacy of using 70% ETOH alone as a
sterilization agent specifically in flow as well as generally in the
laboratory. Although ETOH has some bactericidal activity against common
water-borne contaminants such as the pseudomonads and the like I would
imagine that most airborne contaminants that float into the sheath tank
would be of the Gram-positive persuasion and as such quite resistant to the
effect of ETOH.
As for swabbing down external surfaces with 70% ETOH, I would regard this
as an excellent strategy for mobilizing bacterial aerosol.
On the few occasions when I have had to carry out sterile sorting, my
strategy is as follows:
Clean sort nozzle/flow cell /sheath tank with a proprietary detergent and
rinse in sterile H2O.
Wash the sort collection area down with a proprietary detergent
Bypass the in-line sheath filter, Then flush system with following:
1)H2O
2)Surface active agent e.g. 0.1% DECON 90
3)Sterile H20
4)Sterile sheath fluid
5)Fit new sterile in-line sheath filter.
Regards .
Arnold.
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Arnold Pizzey
Department of Haematology
98 Chenies Mews
London WC1E 6HX
E-mail a.pizzey@ucl.ac.uk.
Voice (0)171 209 6234;
fax (0)171 209 6222.
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web