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I am sorry for this belated response to a question by Georgia M.
Farris. There are two kits available for staining of bacteria.
The first one stains all bacteria (whether viable or dead). The
second kit also stains all bacteria, but it comes with a flask of
beads of known density. By counting the beads (which are much larger
than the bacteria) you know what volume you have analysed and then
you can calculate how many bacteria there were per microliter of
sample.
Their catalog numbers are 7012 (BacLight) and 7277 (BacCount)
respectively. They are avaliable from Molecular Probes, Inc.; P.O.
Box 22010; Eugene; OR 97402; Tel: (541) 465-8300; FAX: (541) 344-6504.
Good luck !
Martin Poot
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web