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>Fix and perm is not the only way, but it is a good and easy way (it is
often recommended in consensus >prototcols)! You can buy it from caltag in
U.S. Most of our customers use this reagent! But it won't work >for TdT
staining!!
I must refute the latter observation that Fix and Perm won't work for
Tdt's. Our clinical lab routinely uses Fix and Perm for surface plus TdT
detection on patient specimen. I've seen the histograms, and they are
beautiful. I'm curious to know what problems were encountered with that
procedure?
Viki Mosiman
Flow Cytometry Core Research Facility
Northwestern University
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web