 |
 |
 |
 |
 |
Some time ago a question came up as to which dye one would use to
stain DNA/RNA with before microinjection of the fluorescent
complex into cells.
This issue has been addressed by Lucas and co-workers (1995) Science
270, 1980: "Selective trafficking of KNOTTED1 homeodomain protein and
its mRNA through plasmodesmata". The authors stained mRNA with the
TOTO-1 dye, microinjected it into tobacco cells and followed mRNA
trafficking from cell to cell.
TOTO-1 is available from Molecular Probes, Inc. (Tel: (5410 465-8300;
FAX: (541) 344-6504); P.O. Box 22010, Eugene, OR 97402, U.S.A.
Martin Poot
|
|
|
|
|
|
CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web